Rabbit SOD (EC) Polyclonal Antibody | anti-SOD3 antibody

SOD (EC) Antibody: ATTO 390

Cat. Num. AAA805569

• Gene Names: SOD3; EC-SOD
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Synonyms: SOD (EC); Polyclonal Antibody; SOD (EC) Antibody: ATTO 390; Extra-cellular superoxide dismutase; SOD3; anti-SOD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Peptide Affinity Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 240

• Applicable Applications for anti-SOD3 antibody: Western Blot (WB)
• Application Notes: 1:1000 for WB
• Conjugate: ATTO 390
• Storage Buffer: PBS pH 7.4; 50% glycerol, 0.09% azide
• Preparation and Storage: -20 degree C

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (EC) Polyclonal Antibody . Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (EC) Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Golgi lumen. Exosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (EC) Antibody. (C) Composite.)

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (EC) Polyclonal Antibody . Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (EC) Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Golgi lumen. Exosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (EC) Antibody. (C) Composite.)

Western Blot (WB)

(Western blot analysis of Human Cervical Cancer cell lysates (HeLa) showing detection of ~35 kDa SOD (EC) protein using Rabbit Anti-SOD (EC) Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Cervical Cancer cell lysates (HeLa). Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SOD (EC) Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~35 kDa. Other Band(s): 50 kDa.)

Related Product Information for anti-SOD3 antibody

Background Info: Identifies a band ~35kD on WB

Scientific Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2" to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).

Product Categories/Family for anti-SOD3 antibody

Oxidative Stress

References

1. Adachi T., et al. (1992) Clin Chim Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. FurukawaY., and O'Halloran T. (2006) Antioxidants & Redo Signaling. 8(5): 6. 4. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988) Free Radical Biol. Med. 5: 377-385. 6. Wispe J.R., et al. (1989) BBA. 994: 30-36. 7. Regan, E. et al. (2005) Arthritis & Rheumatism 52(11): 3479-3491

NCBI and Uniprot Product Information

• NCBI GI #: 118582275
• NCBI GeneID: 6649
• NCBI Accession #: NP_003093.2
• NCBI GenBank Nucleotide #: NM_003102.2
• UniProt Accession #: P08294; Q5U781; Q6FHA2
• Molecular Weight: 25,851 Da
• NCBI Official Full Name: extracellular superoxide dismutase
• NCBI Official Synonym Full Names: superoxide dismutase 3, extracellular
• NCBI Official Symbol: SOD3
• NCBI Official Synonym Symbols: EC-SOD
• NCBI Protein Information: extracellular superoxide dismutase [Cu-Zn]; extracellular superoxide dismutase [Cu-Zn]
• UniProt Protein Name: Extracellular superoxide dismutase [Cu-Zn]
• Protein Family: Extracellular superoxide dismutase
• UniProt Gene Name: SOD3
• UniProt Synonym Gene Names: EC-SOD
• UniProt Entry Name: SODE_HUMAN

NCBI Description

This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. The product of this gene is thought to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM. [provided by RefSeq, Jul 2008]

Uniprot Description

SOD3: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Belongs to the Cu-Zn superoxide dismutase family.

Protein type: Secreted, signal peptide; Secreted; Oxidoreductase; EC 1.15.1.1

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; cytoplasm; extracellular region; trans-Golgi network; nucleus

Molecular Function: heparin binding; protein binding; copper ion binding; zinc ion binding; superoxide dismutase activity

Biological Process: response to reactive oxygen species; response to copper ion; removal of superoxide radicals; response to hypoxia