Rabbit anti-Human SNX6 Polyclonal Antibody | anti-SNX6 antibody

SNX6 (TRAF4-associated Factor 2, Sorting Nexin-6) (Azide free) (HRP)

Cat. Num. AAA6500044

• Gene Names: SNX6; TFAF2; MSTP010
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: SNX6; Polyclonal Antibody; SNX6 (TRAF4-associated Factor 2; Sorting Nexin-6) (Azide free) (HRP); anti-SNX6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SNX6.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SNX6 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Human SNX6 recombinant protein.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SNX6 Antibody in HL-60 cell line lysates (35ug/lane). SNX6 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-SNX6 antibody

Antibodies; SNX (Sorting Nexin) Proteins

References

1. Parks W.T., J. Biol. Chem. 276:19332-19339(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 1488045793
• NCBI GeneID: 58533
• NCBI Accession #: NP_689419
• NCBI GenBank Nucleotide #: NM_152233
• UniProt Accession #: Q9UNH7
• Molecular Weight: 48kDa
• NCBI Official Full Name: sorting nexin-6 isoform b
• NCBI Official Synonym Full Names: sorting nexin 6
• NCBI Official Symbol: SNX6
• NCBI Official Synonym Symbols: TFAF2; MSTP010
• NCBI Protein Information: sorting nexin-6
• UniProt Protein Name: Sorting nexin-6
• Protein Family: Sorting nexin
• UniProt Gene Name: SNX6

NCBI Description

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein associates with the long isoform of the leptin receptor, the transforming growth factor-beta family of receptor serine-threonine kinases, and with receptor tyrosine kinases for platelet-derived growth factor, insulin, and epidermal growth factor. This protein may form oligomeric complexes with family member proteins through interactions of both the PX domain and the coiled coil regions of the molecules. Translocation of this protein from the cytoplasm to the nucleus occurs after binding to proviral integration site 1 protein. This gene results in two transcripts encoding two distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in several stages of intracellular trafficking. Interacts with membranes phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 4,5-bisphosphate (Probable). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex (PubMed:19935774). The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Does not have in vitro vesicle-to-membrane remodeling activity (PubMed:23085988). Involved in retrograde endosome-to-TGN transport of lysosomal enzyme receptor IGF2R (PubMed:17148574). May function as link between transport vesicles and dynactin (Probable). Negatively regulates retrograde transport of BACE1 from the cell surface to the trans-Golgi network (PubMed:20354142). Involved in E-cadherin sorting and degradation; inhibits PIP5K1C isoform 3-mediated E-cadherin degradation (PubMed:24610942). In association with GIT1 involved in EGFR degradation. Promotes lysosomal degradation of CDKN1B (). May contribute to transcription regulation (Probable).