Rabbit anti-Human, Mouse SNW1 Polyclonal Antibody | anti-SNW1 antibody

SNW1 Antibody

Cat. Num. AAA9630621

• Gene Names: SNW1; Bx42; SKIP; Prp45; SKIIP; PRPF45; NCOA-62
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SNW1; Polyclonal Antibody; SNW1 Antibody; Bx 42; Bx42; Homolog of Drosophila BX42; MGC119379; NCOA 62; Nuclear protein SkiP; Nuclear receptor coactivator 62 kD; Nuclear receptor coactivator NCoA 62; Nuclear receptor coactivator NCoA62; Prp 45; Prp45; PRPF 45; PRPF45; Ski interacting protein; SKIIP; SKIP; SNW 1; SNW domain containing 1; SNW domain containing protein 1; SNW1 protein; anti-SNW1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SNW1 Antibody detects endogenous levels of SNW1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SNW1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human SNW1.
• Conjugation: Unconjugated
• Similarity: Belongs to the SNW family.
• Subunit Structure: Identified in the spliceosome C complex. Associates with U4/U6-U5 tri-small nuclear ribonucleoproteins (U4/U6-U5 tri-snRNPs). Interacts SKI, SMAD2, SMAD3, RBPJ, RB1, PABPN1, MAGEA1, SIRT1, FOXN3, U2AF2, PPIL1, DAXX and ATP1B4. Interacts with VDR and RXRA; preferentially associates with VDR:RXRA heterodimers. Interacts with NCOR2. Interacts with MAML1. Interacts with NOTCH1 NICD; the interaction involves multimerized NOTCH1 NICD. Forms a complex with NOTCH1 NICD and MAML1; the association is dissociated by RBPJ. Associates with positive transcription elongation factor b (P-TEFb). Component of the SNARP complex which consists at least of SNIP1, SNW1, THRAP3, BCLAF1 and PNN.(Microbial infection) Interacts with human papillomavirus type-16 (HPV16) E7 protein.(Microbial infection) Interacts with EBV EBNA2; EBNA2 competes with NCOR2 for interaction with SNW1.
• Function: Involved in pre-mRNA splicing as component of the spliceosome. Is required in the specific splicing of CDKN1A pre-mRNA; the function probably involves the recruitment of U2AF2 to the mRNA. Is proposed to recruit PPIL1 to the spliceosome. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. Involved in transcriptional regulation. Modulates TGF-beta-mediated transcription via association with SMAD proteins, MYOD1-mediated transcription via association with PABPN1, RB1-mediated transcriptional repression, and retinoid-X receptor (RXR)- and vitamin D receptor (VDR)-dependent gene transcription in a cell line-specific manner probably involving coactivators NCOA1 and GRIP1. Is involved in NOTCH1-mediated transcriptional activation. Binds to multimerized forms of Notch intracellular domain (NICD) and is proposed to recruit transcriptional coactivators such as MAML1 to form an intermediate preactivation complex which associates with DNA-bound CBF-1/RBPJ to form a transcriptional activation complex by releasing SNW1 and redundant NOTCH1 NICD.(Microbial infection) Is recruited by HIV-1 Tat to Tat:P-TEFb:TAR RNA complexes and is involved in Tat transcription by recruitment of MYC, MEN1 and TRRAP to the HIV promoter.(Microbial infection) Proposed to be involved in transcriptional activation by EBV EBNA2 of CBF-1/RBPJ-repressed promoters.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-SNW1 antibody

Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 6912676
• NCBI GeneID: 22938
• NCBI Accession #: NP_036377
• NCBI GenBank Nucleotide #: NM_012245.2
• UniProt Accession #: Q13573; Q13483; Q32N03; Q5D0D6; A8K8A9
• Molecular Weight: 61,494 Da
• NCBI Official Full Name: SNW domain-containing protein 1
• NCBI Official Synonym Full Names: SNW domain containing 1
• NCBI Official Symbol: SNW1
• NCBI Official Synonym Symbols: Bx42; SKIP; Prp45; SKIIP; PRPF45; NCOA-62
• NCBI Protein Information: SNW domain-containing protein 1; nuclear protein SkiP; SKI interacting protein; SKI-interacting protein; homolog of Drosophila BX42; nuclear receptor coactivator, 62-kD; nuclear receptor coactivator NCoA-62
• UniProt Protein Name: SNW domain-containing protein 1
• Protein Family: SNW domain-containing protein
• UniProt Gene Name: SNW1
• UniProt Synonym Gene Names: SKIIP; SKIP
• UniProt Entry Name: SNW1_HUMAN

NCBI Description

This gene, a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also function as a splicing factor by interacting with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity. [provided by RefSeq, Jul 2008]

Uniprot Description

Skip: Involved in vitamin D-mediated transcription. Can function as a splicing factor in pre-mRNA splicing. Belongs to the SNW family.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Spliceosome

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: nucleoplasm; spliceosome; transcription elongation factor complex b; nuclear matrix; chromatin; nucleus

Molecular Function: protein binding; vitamin D receptor binding; retinoic acid receptor binding; transcription coactivator activity; Notch binding; SMAD binding; transcription corepressor activity; nuclear hormone receptor binding

Biological Process: retinoic acid receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; positive regulation of neurogenesis; viral reproduction; positive regulation of transforming growth factor beta receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; positive regulation of nuclear mRNA splicing, via spliceosome; nuclear mRNA splicing, via spliceosome; regulation of transcription from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; regulation of retinoic acid receptor signaling pathway