Rabbit SNURF Polyclonal Antibody | anti-SNURF antibody

SNURF Antibody (Center)

Cat. Num. AAA9211423

• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rabbit)
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: SNURF; Polyclonal Antibody; SNURF Antibody (Center); SNRPN upstream reading frame protein; anti-SNURF antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rabbit)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This SNURF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the Central region of human SNURF.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 4-32
• Sequence Length: 71

• Applicable Applications for anti-SNURF antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: WB~~1:1000
• Cellular location: Nucleus
• Tissue location: Expressed in heart, skelteal muscle and lymphoblast (at protein level). Expressed in brain, pancreas, heart , liver, lung, kidney and skeletal muscle.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-SNURF Antibody (Center) in Hela cell line lysates (35ug/lane). SNURF(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with SNURF Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SNURF Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SNURF antibody

SNURF is a highly basic protein localized to the nucleus. The evolutionarily constrained open reading frame of its gene is found on a bicistronic transcript which has a downstream ORF encoding the small nuclear ribonucleoprotein polypeptide N. The upstream coding region utilizes the first three exons of the transcript, a region that has been identified as an imprinting center. Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region but the full-length nature of these transcripts has not been determined. An alternate exon has been identified that substitutes for exon 4 and leads to a truncated, monocistronic transcript. Alternative splicing or deletion caused by a translocation event in the 5' UTR or coding region of this gene leads to Angelman syndrome or Prader-Willi syndrome due to parental imprint switch failure. The function of this protein is not yet known.

Product Categories/Family for anti-SNURF antibody

Cell Biology

References

Rodriguez-Jato,S., Nucleic Acids Res. 33 (15), 4740-4753 (2005)
Runte,M., Hum. Genet. 114 (6), 553-561 (2004)
Runte,M., Hum. Mol. Genet. 10 (23), 2687-2700 (2001)

NCBI and Uniprot Product Information

• NCBI GI #: 13027653
• NCBI GeneID: 8926
• NCBI Accession #: NP_005669.2; NP_073715.1
• NCBI GenBank Nucleotide #: NM_005678.3
• UniProt Accession #: Q9Y675; A6NCW2
• NCBI Official Full Name: SNRPN upstream reading frame protein
• NCBI Official Synonym Full Names: SNRPN upstream reading frame
• NCBI Official Symbol: SNURF
• NCBI Protein Information: SNRPN upstream reading frame protein
• UniProt Protein Name: SNRPN upstream reading frame protein
• Protein Family: SNRPN upstream reading frame protein
• UniProt Gene Name: SNURF
• UniProt Entry Name: SNURF_HUMAN

NCBI Description

This gene encodes a highly basic protein localized to the nucleus. The evolutionarily constrained open reading frame is found on a bicistronic transcript which has a downstream ORF encoding the small nuclear ribonucleoprotein polypeptide N. The upstream coding region utilizes the first three exons of the transcript, a region that has been identified as an imprinting center. Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region but the full-length nature of these transcripts has not been determined. An alternate exon has been identified that substitutes for exon 4 and leads to a truncated, monocistronic transcript. Alternative splicing or deletion caused by a translocation event in the 5' UTR or coding region of this gene leads to Angelman syndrome or Prader-Willi syndrome due to parental imprint switch failure. The function of this protein is not yet known. [provided by RefSeq, Jul 2008]

Uniprot Description

SNURF: a highly basic 71-aa nuclear protein. Encoded with SNRPN on a polycistronic transcript. Play an important role in maintaining differentialy methylated DNA. The gene is imprinted, and disruption of imprinting plays a role in Prader-Willi syndrome (PWS). Translated in normal, but not PWS, human, and mouse tissues.

Protein type: Transcription regulation; Gene silencing

Chromosomal Location of Human Ortholog: 15q12

Cellular Component: nucleus