SNuRF recombinant protein

SNuRF, 1-71aa, Human, His tag, E Coli

Cat. Num. AAA205593

• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: SNuRF; 1-71aa; Human; His tag; E Coli; SNRPN upstream reading frame protein; Small nuclear ring finger protein; SNuRF recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 40% glycerol, 1mM DTT.
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMERARDR LHLRRTTEQH VPEVEVQVKR RRTASLSNQE CQLYPRRSQQ QQVPVVDFQA ELRQAFLAET PRGG
• Sequence Length: 71

• Applicable Applications for SNuRF recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for SNuRF recombinant protein

SNuRF is a highly basic protein localized to the nucleus. The evolutionarily constrained open reading frame is found on a bicistronic transcript which has a downstream ORF encoding the small nuclear ribonucleoprotein polypeptide N. The upstream coding region utilizes the first three exons of the transcript, a region that has been identified as an imprinting center. Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region but the full-length nature of these transcripts has not been determined. An alternate exon has been identified that substitutes for exon 4 and leads to a truncated, monocistronic transcript. Alternative splicing or deletion caused by a translocation event in the 5' uTR or coding region of this gene leads to Angelman syndrome or Prader-Willi syndrome due to parental imprint switch failure. The function of this protein is not yet known. Recombinant human SNuRF protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

References

Runte M, Kroisel PM et al. (2004). Hum Genet. 114 (6):553-61.

NCBI and Uniprot Product Information

• NCBI GI #: 13027655
• NCBI GeneID: 8926
• NCBI Accession #: NP_073715
• NCBI GenBank Nucleotide #: NM_022804.2
• Molecular Weight: 10.8 kDa (94aa) confirmed by MALDI-TOF
• NCBI Official Full Name: SNRPN upstream reading frame protein
• NCBI Official Synonym Full Names: SNRPN upstream reading frame
• NCBI Official Symbol: SNURF
• NCBI Protein Information: SNRPN upstream reading frame protein
• UniProt Protein Name: SNRPN upstream reading frame protein
• Protein Family: SNRPN upstream reading frame protein
• UniProt Gene Name: SNURF

NCBI Description

This gene is located within the Prader-Willi Syndrome critical region on chromosome 15. Transcripts produced from this gene initiate at an imprinting center and are paternally-imprinted. These transcripts may be bicistronic and also encode SNRPN (small nuclear ribonucleoprotein polypeptide N) from a downstream open reading frame. The small protein represented by this gene is encoded by an evolutionarily-conserved upstream open reading frame and is localized to the nucleus. Extensive alternative splicing and promoter usage occurs in this region and the full-length nature of some of these transcripts has not been determined. Alterations in the imprinting center are associated with parental imprint switch failure, which may cause Angelman syndrome or Prader-Willi syndrome. [provided by RefSeq, Mar 2017]

Uniprot Description

MiscellaneousEncoded on a bicistronic transcript that code for two proteins, SNRPN and SNURF. In addition to the primary 1.6-kb bicistronic SNURF-SNRPN transcript, SNURF-only transcript is also detected.