SNRPN Polyclonal Antibody | anti-SNRPN antibody

Anti-SNRPN Antibody

Cat. Num. AAA177823

• Gene Names: SNRPN; SMN; PWCR; SM-D; sm-N; RT-LI; HCERN3; SNRNP-N; SNURF-SNRPN
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: SNRPN; Polyclonal Antibody; Anti-SNRPN Antibody; Small nuclear ribonucleoprotein-associated protein N; HCERN3; PWCR; RSMN_HUMAN; RT LI; RTLI; SM D; Sm N; Sm protein D; Sm protein N; Sm-D; Sm-N; Small nuclear ribonucleoprotein associated protein N; Small nuclear ribonucleoprotein polypeptide N; SMD; SmN; SNRNP N; snRNP-N; SNRNPN; SNURF SNRPN; Tissue specific splicing protein; Tissue-specific-splicing protein antibody; small nuclear ribonucleoprotein polypeptide N; anti-SNRPN antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 240

• Applicable Applications for anti-SNRPN antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SNRPN (11-39aa QHIDYRMRCILQDGRIFIGTFKAFDKHMN), identical to the related mouse and rat sequences.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- SNRPN Picoband antibody, MBS177823, Western blottingAll lanes: Anti SNRPN (MBS177823) at 0.5ug/mlLane 1: Rat Thymus Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: HEPG2 Whole Cell Lysate at 40ugLane 4: HEPA Whole Cell Lysate at 40ugPredicted bind size: 25KDObserved bind size: 32KD )

Immunohistochemistry (IHC)

(Anti- SNRPN Picoband antibody, MBS177823, IHC(P)IHC(P): Mouse Brain Tissue )

Immunohistochemistry (IHC)

(Anti- SNRPN Picoband antibody, MBS177823, IHC(P)IHC(P): Rat Thymus Tissue )

Immunohistochemistry (IHC)

(Anti- SNRPN Picoband antibody, MBS177823, IHC(P)IHC(P): Human Tonsil Tissue )

Related Product Information for anti-SNRPN antibody

Description: Rabbit IgG polyclonal antibody for Small nuclear ribonucleoprotein-associated protein N(SNRPN) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: SNRPN(Small Nuclear Ribonucleoprotein Polypeptide N), also called SMN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide. The protein encoded by this gene is one polypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family. SNRPN also encodes a long alternatively spliced transcript containing several small nucleolar RNAs (snoRNAs) and extends downstream to partially overlap the UBE3A gene in the antisense orientation. PWS arises from loss of function of genes in this region expressed exclusively from the paternal chromosome, suggesting that SNRPN may play a role in its etiology. The SNRPN gene is mapped on 15q11.2. Analysis of maternal DNA and of SNRPN cDNA confirmed that the maternal allele is not expressed in fetal brain and heart. Deletions in the transcription unit of the imprinted SNRPN gene occur in patients who have PWS or Angelman syndrome because of a parental imprint switch failure in this chromosomal domain.

References

1. Bielinska, B., Blaydes, S. M., Buiting, K., Yang, T., Krajewska-Walasek, M., Horsthemke, B., Brannan, C. I. De novo deletions of SNRPN exon 1 in early human and mouse embryos result in a paternal to maternal imprint switch. Nature Genet. 25: 74-78, 2000. 2. Cattanach, B. M., Barr, J. A., Evans, E. P., Burtenshaw, M., Beechey, C. V., Leff, S. E., Brannan, C. I., Copeland, N. G., Jenkins, N. A., Jones, J. A candidate mouse model for Prader-Willi syndrome which shows an absence of Snrpn expression. Nature Genet. 2: 270-274, 1992. 3. Geuns, E., De Rycke, M., Van Steirteghem, A., Liebaers, I. Methylation imprints of the imprint control region of the SNRPN-gene in human gametes and preimplantation embryos. Hum. Molec. Genet. 12: 2873-2879, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 4507135
• NCBI GeneID: 6638
• NCBI Accession #: NP_003088.1
• NCBI GenBank Nucleotide #: NM_003097.4
• UniProt Accession #: P63162; P14648; P17135; Q0D2Q5; B3KVR1
• Molecular Weight: 25,076 Da
• NCBI Official Full Name: small nuclear ribonucleoprotein-associated protein N
• NCBI Official Synonym Full Names: small nuclear ribonucleoprotein polypeptide N
• NCBI Official Symbol: SNRPN
• NCBI Official Synonym Symbols: SMN; PWCR; SM-D; sm-N; RT-LI; HCERN3; SNRNP-N; SNURF-SNRPN
• NCBI Protein Information: small nuclear ribonucleoprotein-associated protein N
• UniProt Protein Name: Small nuclear ribonucleoprotein-associated protein N
• Protein Family: SNRPN upstream reading frame protein
• UniProt Gene Name: SNRPN
• UniProt Synonym Gene Names: HCERN3; SMN; snRNP-N; Sm-D; Sm-N; SmN
• UniProt Entry Name: RSMN_HUMAN

NCBI Description

The protein encoded by this gene is one polypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family. The protein plays a role in pre-mRNA processing, possibly tissue-specific alternative splicing events. Although individual snRNPs are believed to recognize specific nucleic acid sequences through RNA-RNA base pairing, the specific role of this family member is unknown. The protein arises from a bicistronic transcript that also encodes a protein identified as the SNRPN upstream reading frame (SNURF). Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region. Additional splice variants have been described but sequences for the complete transcripts have not been determined. The 5' UTR of this gene has been identified as an imprinting center. Alternative splicing or deletion caused by a translocation event in this paternally-expressed region is responsible for Angelman syndrome or Prader-Willi syndrome due to parental imprint switch failure. [provided by RefSeq, Jul 2008]

Uniprot Description

snRNP N: May be involved in tissue-specific alternative RNA processing events. Belongs to the snRNP SmB/SmN family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 15q11.2

Cellular Component: cytoplasm; small nuclear ribonucleoprotein complex; snRNP U1; snRNP U2; snRNP U5; spliceosome; U4/U6 x U5 tri-snRNP complex

Molecular Function: protein binding; RNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; response to hormone stimulus; RNA splicing

Disease: Autism; Prader-willi Syndrome