Rabbit anti-Human SNRPN Polyclonal Antibody | anti-SNRPN antibody

SNRPN, NT (SNRPN, HCERN3, SMN, Small nuclear ribonucleoprotein-associated protein N, Sm protein D, Sm protein N, Tissue-specific-splicing protein) (FITC)

Cat. Num. AAA6349444

• Gene Names: SNRPN; SMN; PWCR; SM-D; sm-N; RT-LI; HCERN3; SNRNP-N; SNURF-SNRPN
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SNRPN; Polyclonal Antibody; NT (SNRPN; HCERN3; SMN; Small nuclear ribonucleoprotein-associated protein N; Sm protein D; Sm protein N; Tissue-specific-splicing protein) (FITC); Tissue-specific-splicing protein; anti-SNRPN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 240

• Applicable Applications for anti-SNRPN antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SNRPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human SNRPN.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SNRPN Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SNRPN antibody detected the SNRPN protein (arrow).)

Immunohistochemistry (IHC)

(SNRPN Antibody (N-term) (AP14532a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SNRPN Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SNRPN antibody

The protein encoded by this gene is one polypeptide of a small nuclear ribonucleoprotein complex and belongs to the snRNP SMB/SMN family. The protein plays a role in pre-mRNA processing, possibly tissue-specific alternative splicing events. Although individual snRNPs are believed to recognize specific nucleic acid sequences through RNA-RNA base pairing, the specific role of this family member is unknown. The protein arises from a bicistronic transcript that also encodes a protein identified as the SNRPN upstream reading frame (SNURF). Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region. Additional splice variants have been described but sequences for the complete transcripts have not been determined. The 5' UTR of this gene has been identified as an imprinting center. Alternative splicing or deletion caused by a translocation event in this paternally-expressed region is responsible for Angelman syndrome or Prader-Willi syndrome due to parental imprint switch failure.

Product Categories/Family for anti-SNRPN antibody

Antibodies; Nuclear Proteins

References

Benetatos, L., et al. Leuk. Res. 34(2):148-153(2010). Kim, S.J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (7), 1116-1125 (2008): Horsthemke, B., et al. Am. J. Med. Genet. A 146A (16), 2041-2052 (2008): Runte, M., et al. Hum. Mol. Genet. 10(23):2687-2700(2001). Kuslich, C.D., et al. Am. J. Hum. Genet. 64(1):70-76(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 13027644
• NCBI GeneID: 6638
• NCBI Accession #: NP_073716.1; NP_073717.1
• NCBI GenBank Nucleotide #: NP_073716.1
• UniProt Accession #: P63162
• NCBI Official Full Name: small nuclear ribonucleoprotein-associated protein N isoform b
• NCBI Official Synonym Full Names: small nuclear ribonucleoprotein polypeptide N
• NCBI Official Symbol: SNRPN
• NCBI Official Synonym Symbols: SMN; PWCR; SM-D; sm-N; RT-LI; HCERN3; SNRNP-N; SNURF-SNRPN
• NCBI Protein Information: small nuclear ribonucleoprotein-associated protein N
• UniProt Protein Name: Small nuclear ribonucleoprotein-associated protein N
• Protein Family: SNRPN upstream reading frame protein
• UniProt Gene Name: SNRPN
• UniProt Synonym Gene Names: HCERN3; SMN; snRNP-N; Sm-D; Sm-N; SmN
• UniProt Entry Name: RSMN_HUMAN

NCBI Description

This gene is located within the Prader-Willi Syndrome critical region on chromosome 15 and is imprinted and expressed from the paternal allele. It encodes a component of the small nuclear ribonucleoprotein complex, which functions in pre-mRNA processing and may contribute to tissue-specific alternative splicing. Alternative promoter use and alternative splicing result in a multitude of transcript variants encoding the same protein. Transcript variants that initiate at the CpG island-associated imprinting center may be bicistronic and also encode the SNRPN upstream reading frame protein (SNURF) from an upstream open reading frame. In addition, long spliced transcripts for small nucleolar RNA host gene 14 (SNHG14) may originate from the promoters at this locus and share exons with this gene. Alterations in this region are associated with parental imprint switch failure, which may cause Angelman syndrome or Prader-Willi syndrome. [provided by RefSeq, Mar 2017]

Uniprot Description

snRNP N: May be involved in tissue-specific alternative RNA processing events. Belongs to the snRNP SmB/SmN family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 15q11.2

Cellular Component: small nuclear ribonucleoprotein complex; spliceosome; snRNP U2; snRNP U1

Molecular Function: protein binding; RNA binding

Biological Process: RNA splicing; response to hormone stimulus

Disease: Prader-willi Syndrome; Autism