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Western Blot (WB) (Western Blot (WB) analysis of SNRPD3 polyclonal antibody at 1:500 dilutionLane1:Jurkat whole cell lysateLane2:Mouse brain tissue lysateLane3:H9C2 whole cell lysate)

Rabbit SNRPD3 Polyclonal Antibody | anti-SNRPD3 antibody

SNRPD3 Polyclonal Antibody

Gene Names
SNRPD3; SMD3; Sm-D3
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SNRPD3; Polyclonal Antibody; SNRPD3 Polyclonal Antibody; Small nuclear ribonucleoprotein Sm D3; Sm-D3; snRNP core protein D3; anti-SNRPD3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SNRPD3 polyclonal antibody detects endogenous levels of SNRPD3 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
126
Applicable Applications for anti-SNRPD3 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human SNRPD3.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SNRPD3 polyclonal antibody at 1:500 dilutionLane1:Jurkat whole cell lysateLane2:Mouse brain tissue lysateLane3:H9C2 whole cell lysate)

Western Blot (WB) (Western Blot (WB) analysis of SNRPD3 polyclonal antibody at 1:500 dilutionLane1:Jurkat whole cell lysateLane2:Mouse brain tissue lysateLane3:H9C2 whole cell lysate)
Related Product Information for anti-SNRPD3 antibody
U1, U2, U4, U5, U6 and U7 are small nuclear ribonucleoproteins (snRNPs) that comprise the spliceosome in eukaryotes. Each UsnRNP contains common Sm proteins B/B', D1, D2, D3, E, F and G. The Sm proteins pair up as D1-D2, B/B'-D3 and E-F-G to form RNA-free hetero-oligomers in the cytoplasm. Sm proteins aid in the cytoplasmic construction of the UsnRNPs by binding to a conserved Sm site on UsnRNA and forming a stable snRNP core complex. Sm D1, D2 and D3 are present in U1, U2, U4/5 and U5 but not U7 snRNPs in human and mouse cells. U7 snRNPs contain Lsm10, an Sm D1-like protein. Autoantibodies produced in patients suffering from systemic lupus erythematosus react predominantly with Sm B/B', D1 and D3. The major linear epitope of these autoantibodies includes the C-terminal RG dipeptide repeats found in Sm D1 and D3.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 14kDa
NCBI Official Full Name
small nuclear ribonucleoprotein Sm D3
NCBI Official Synonym Full Names
small nuclear ribonucleoprotein D3 polypeptide
NCBI Official Symbol
SNRPD3
NCBI Official Synonym Symbols
SMD3; Sm-D3
NCBI Protein Information
small nuclear ribonucleoprotein Sm D3
UniProt Protein Name
Small nuclear ribonucleoprotein Sm D3
UniProt Gene Name
SNRPD3
UniProt Synonym Gene Names
Sm-D3
UniProt Entry Name
SMD3_HUMAN

NCBI Description

This gene encodes a core component of the spliceosome, which is a nuclear ribonucleoprotein complex that functions in pre-mRNA splicing. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

snRNP D3: Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner. Belongs to the snRNP core protein family.

Protein type: Spliceosome; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: small nuclear ribonucleoprotein complex; nucleoplasm; spliceosome; cytoplasm; snRNP U1; cytosol; U12-dependent spliceosome

Molecular Function: protein binding; enzyme binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; spliceosomal snRNP biogenesis; RNA splicing; histone mRNA metabolic process; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription

Research Articles on SNRPD3

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Product Notes

The SNRPD3 snrpd3 (Catalog #AAA3006337) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SNRPD3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SNRPD3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the SNRPD3 snrpd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNRPD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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