Human CD27 Binding Protein (CD27BP) ELISA Kit | CD27BP elisa kit

Human CD27 Binding Protein (CD27BP) ELISA Kit

Cat. Num. AAA2707059

• Gene Names: CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
• Reactivity: Human
• Synonyms: CD27 Binding Protein (CD27BP); Human CD27 Binding Protein (CD27BP) ELISA Kit; SIVA; SIVA1; Apoptosis-Inducing Factor; Apoptosis regulatory protein Siva; CD27BP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CD27 Binding Protein (CD27BP).; No significant cross-reactivity or interference between CD27 Binding Protein (CD27BP) and analogues was observed.
• Sequence Length: 260

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.068ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CD27 Binding Protein (CD27BP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CD27 Binding Protein (CD27BP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD27BP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD27 Binding Protein (CD27BP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CD27 Binding Protein (CD27BP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD27 Binding Protein (CD27BP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD27 Binding Protein (CD27BP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507587
• NCBI GeneID: 939
• NCBI Accession #: NP_001233.1
• NCBI GenBank Nucleotide #: NM_001242.4
• UniProt Accession #: P26842; B2RDZ0
• Molecular Weight: 29,137 Da
• NCBI Official Full Name: CD27 antigen
• NCBI Official Synonym Full Names: CD27 molecule
• NCBI Official Symbol: CD27
• NCBI Official Synonym Symbols: T14; S152; Tp55; TNFRSF7; S152. LPFS2
• NCBI Protein Information: CD27 antigen
• UniProt Protein Name: CD27 antigen
• UniProt Gene Name: CD27
• UniProt Synonym Gene Names: TNFRSF7

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.