Rabbit anti-Human SNRPD1 Polyclonal Antibody | anti-SNRPD1 antibody

SNRPD1, ID (Small Nuclear Ribonucleoprotein Sm D1, Sm-D1, snRNP Core Protein D1, Sm-D Autoantigen) (HRP)

Cat. Num. AAA6499989

• Gene Names: SNRPD1; SMD1; SNRPD; Sm-D1; HsT2456
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: SNRPD1; Polyclonal Antibody; ID (Small Nuclear Ribonucleoprotein Sm D1; Sm-D1; snRNP Core Protein D1; Sm-D Autoantigen) (HRP); anti-SNRPD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SNRPD1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SNRPD1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human SNRPD1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of anti-SNRPD1 Antibody (Center) in HL60 cell line lysates (35ug/lane). SNRPD1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with MASTL Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of SNRPD1 (arrow) using rabbit polyclonal SNRPD1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SNRPD1 gene.)

Flow Cytometry (FC/FACS)

(SNRPD1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SNRPD1 antibody

Antibodies; Nuclear Proteins

References

1. Lehner, B., Genome Res. 14(7), 1315-1323 (2004). 2. Will, C.L., RNA 10(6), 929-941 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 5902102
• NCBI GeneID: 6632
• NCBI Accession #: NP_008869
• NCBI GenBank Nucleotide #: NM_006938
• UniProt Accession #: P62314
• Molecular Weight: 13kDa
• NCBI Official Full Name: small nuclear ribonucleoprotein Sm D1 isoform 1
• NCBI Official Synonym Full Names: small nuclear ribonucleoprotein D1 polypeptide
• NCBI Official Symbol: SNRPD1
• NCBI Official Synonym Symbols: SMD1; SNRPD; Sm-D1; HsT2456
• NCBI Protein Information: small nuclear ribonucleoprotein Sm D1
• UniProt Protein Name: Small nuclear ribonucleoprotein Sm D1
• Protein Family: Small nuclear ribonucleoprotein
• UniProt Gene Name: SNRPD1
• UniProt Synonym Gene Names: Sm-D1
• UniProt Entry Name: SMD1_HUMAN

NCBI Description

This gene encodes a small nuclear ribonucleoprotein that belongs to the SNRNP core protein family. The protein may act as a charged protein scaffold to promote SNRNP assembly or strengthen SNRNP-SNRNP interactions through nonspecific electrostatic contacts with RNA. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]

Uniprot Description

snRNP D1: May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through nonspecific electrostatic contacts with RNA. Belongs to the snRNP core protein family.

Protein type: Spliceosome; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: small nuclear ribonucleoprotein complex; nucleoplasm; snRNP U1; cytosol; nucleus; U12-dependent spliceosome

Molecular Function: protein binding; RNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; spliceosomal snRNP biogenesis; RNA splicing; spliceosome assembly; gene expression