Rabbit anti-Human SNRPB Polyclonal Antibody | anti-SNRPB antibody

SNRPB, NT (R49) (Small Nuclear Ribonucleoprotein-associated Proteins B and B', snRNP-B, Sm Protein B/B', Sm-B/Sm-B', SmB/SmB', SNRPB1, COD) (MaxLight 750)

Cat. Num. AAA6500021

• Gene Names: SNRPB; COD; CCMS; SNRPB1; SmB/B'; Sm-B/B'; snRNP-B; SmB/SmB'
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: SNRPB; Polyclonal Antibody; NT (R49) (Small Nuclear Ribonucleoprotein-associated Proteins B and B'; snRNP-B; Sm Protein B/B'; Sm-B/Sm-B'; SmB/SmB'; SNRPB1; COD) (MaxLight 750); anti-SNRPB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SNRPB.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
• Sequence Length: 240

• Applicable Applications for anti-SNRPB antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human SNRPB (KLH).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SNRPB Antibody (N-term R49) in T47D cell line lysates (35ug/lane). SNRPB (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(SNRPB Antibody (N-term R49) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SNRPB antibody

Antibodies; Nuclear Proteins

References

1. Elkon, K.B., et.al., J. Immunol. 145(2), 636-643 (1990). 2. Chu, J.L. et.al., Gene 97(2), 311-312 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 38150007
• NCBI GeneID: 6628
• NCBI Accession #: NP_937859
• NCBI GenBank Nucleotide #: NP_937859.1
• UniProt Accession #: P14678
• Molecular Weight: 24.61
• NCBI Official Full Name: small nuclear ribonucleoprotein-associated proteins B and B' isoform B'
• NCBI Official Synonym Full Names: small nuclear ribonucleoprotein polypeptides B and B1
• NCBI Official Symbol: SNRPB
• NCBI Official Synonym Symbols: COD; CCMS; SNRPB1; SmB/B'; Sm-B/B'; snRNP-B; SmB/SmB'
• NCBI Protein Information: small nuclear ribonucleoprotein-associated proteins B and B'
• UniProt Protein Name: Small nuclear ribonucleoprotein-associated proteins B and B'
• Protein Family: Small nuclear ribonucleoprotein-associated protein
• UniProt Gene Name: SNRPB
• UniProt Synonym Gene Names: COD; SNRPB1; snRNP-B; Sm-B/B'; SmB/B'
• UniProt Entry Name: RSMB_HUMAN

NCBI Description

The protein encoded by this gene is one of several nuclear proteins that are found in common among U1, U2, U4/U6, and U5 small ribonucleoprotein particles (snRNPs). These snRNPs are involved in pre-mRNA splicing, and the encoded protein may also play a role in pre-mRNA splicing or snRNP structure. Autoantibodies from patients with systemic lupus erythematosus frequently recognize epitopes on the encoded protein. Two transcript variants encoding different isoforms (B and B') have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

snRNP B1: Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. May have a functional role in the pre-mRNA splicing or in snRNP structure. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner. Belongs to the snRNP SmB/SmN family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; Spliceosome; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: small nuclear ribonucleoprotein complex; nucleoplasm; spliceosome; snRNP U1; cytosol; U12-dependent spliceosome

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; spliceosomal snRNP biogenesis; RNA splicing; histone mRNA metabolic process; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription