Rabbit anti-Human, Mouse SNRPA Polyclonal Antibody | anti-SNRPA antibody

SNRPA (U1 Small Nuclear Ribonucleoprotein A, U1 snRNP A, U1-A, U1A) APC

Cat. Num. AAA6394620

• Gene Names: SNRPA; U1A; Mud1; U1-A
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SNRPA; Polyclonal Antibody; SNRPA (U1 Small Nuclear Ribonucleoprotein A; U1 snRNP A; U1-A; U1A) APC; anti-SNRPA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SNRPA. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-SNRPA antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human SNRPA, aa1-282 (NP_004587.1).
• Immunogen Sequence: MAVPETRPNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGTFVERDRKREKRKPKSQETPATKKAVQGGGATPVVGAVQGPVPGMPPMTQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQLMPGQMPPAQPLSENPPNHILFLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAGAARDALQGFKITQNNAMKISFAKK
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SNRPA rabbit polyclonal antibody. Western Blot analysis of SNRPA expression in human kidney.)

Western Blot (WB)

(SNRPA rabbit polyclonal antibody. Western Blot analysis of SNRPA expression in mouse kidney.)

Western Blot (WB)

(Western Blot analysis of SNRPA expression in transfected 293T cell line by SNRPA polyclonal antibody. Lane 1: SNRPA transfected lysate (31.3kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-SNRPA antibody

Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process.

Product Categories/Family for anti-SNRPA antibody

Antibodies; Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4759156
• NCBI GeneID: 6626
• NCBI Accession #: NP_004587.1
• NCBI GenBank Nucleotide #: NM_004596
• UniProt Accession #: P09012
• Molecular Weight: 31,280 Da
• NCBI Official Full Name: U1 small nuclear ribonucleoprotein A
• NCBI Official Synonym Full Names: small nuclear ribonucleoprotein polypeptide A
• NCBI Official Symbol: SNRPA
• NCBI Official Synonym Symbols: U1A; Mud1; U1-A
• NCBI Protein Information: U1 small nuclear ribonucleoprotein A; U1 snRNP A; U1 snRNP-specific protein A; U1 small nuclear RNP-specific A
• UniProt Protein Name: U1 small nuclear ribonucleoprotein A
• Protein Family: U1 small nuclear ribonucleoprotein
• UniProt Gene Name: SNRPA
• UniProt Synonym Gene Names: U1 snRNP A; U1-A; U1A
• UniProt Entry Name: SNRPA_HUMAN

NCBI Description

The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation. [provided by RefSeq, Oct 2010]

Uniprot Description

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.