Rabbit anti-Human SNIP1 Polyclonal Antibody | anti-SNIP1 antibody

SNIP1, NT (SNIP1, Smad nuclear-interacting protein 1, FHA domain-containing protein SNIP1) (PE)

Cat. Num. AAA6349330

• Gene Names: SNIP1; PMRED
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SNIP1; Polyclonal Antibody; NT (SNIP1; Smad nuclear-interacting protein 1; FHA domain-containing protein SNIP1) (PE); FHA domain-containing protein SNIP1; anti-SNIP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SNIP1 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SNIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-118 amino acids from the N-terminal region of human SNIP1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SNIP1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SNIP1 antibody detected the SNIP1 protein (arrow).)

Flow Cytometry (FC/FACS)

(SNIP1 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SNIP1 antibody

Down-regulates NF-kappa-B signaling by competing with RELA for CREBBP/EP300 binding. SNIP1 is involved in the microRNA (miRNA) biogenesis.

Product Categories/Family for anti-SNIP1 antibody

Antibodies; Smad Proteins

References

Bracken, C.P., et al. Cancer Res. 68(18):7621-7628(2008). Yu, B., et al. Proc. Natl. Acad. Sci. U.S.A. 105(29):10073-10078(2008). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 21314720
• NCBI GeneID: 79753
• NCBI Accession #: NP_078976.2
• NCBI GenBank Nucleotide #: NM_024700.3
• UniProt Accession #: Q8TAD8; Q96SP9; Q9H9T7
• Molecular Weight: 45,778 Da
• NCBI Official Full Name: smad nuclear-interacting protein 1
• NCBI Official Synonym Full Names: Smad nuclear interacting protein 1
• NCBI Official Symbol: SNIP1
• NCBI Official Synonym Symbols: PMRED
• NCBI Protein Information: smad nuclear-interacting protein 1; FHA domain-containing protein SNIP1
• UniProt Protein Name: Smad nuclear-interacting protein 1
• Protein Family: Smad nuclear interacting protein
• UniProt Gene Name: SNIP1
• UniProt Entry Name: SNIP1_HUMAN

NCBI Description

This gene encodes a protein that contains a coiled-coil motif and C-terminal forkhead-associated (FHA) domain. The encoded protein functions as a transcriptional coactivator that increases c-Myc activity and inhibits transforming growth factor beta (TGF-beta) and nuclear factor kappa-B (NF-kB) signaling. The encoded protein also regulates the stability of cyclin D1 mRNA, and may play a role in cell proliferation and cancer progression. Mutations in this gene are a cause of psychomotor retardation, epilepsy, and craniofacial dysmorphism (PMRED). [provided by RefSeq, Mar 2012]

Uniprot Description

SNIP1: Down-regulates NF-kappa-B signaling by competing with RELA for CREBBP/EP300 binding. Involved in the microRNA (miRNA) biogenesis. Defects in SNIP1 are the cause of psychomotor retardation, epilepsy, and craniofacial dysmorphism (PMRED). A disease characterized by severe psychomotor retardation, intractable seizures, dysmorphic features, and a lumpy skull surface. Patients are hypotonic and have poor feeding in the neonatal period.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding

Biological Process: miRNA-mediated gene silencing, production of miRNAs; I-kappaB kinase/NF-kappaB cascade; regulation of transcription, DNA-dependent

Disease: Psychomotor Retardation, Epilepsy, And Craniofacial Dysmorphism