Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with GTF2I Antibody (C-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).GTF2I immunoreactivity is localized to Nucleus significantly.)
Rabbit anti-Human TFII-I Polyclonal Antibody | anti-TFII-I antibody
TFII-I, CT (Transcription Factor II-I, Bruton Tyrosine Kinase-associated Protein 135, BTK-associated Protein 135, BTK-associated Protein 135kD, BAP135, BAP-135, BTK-associated Protein, BTKAP1, DIWS, General Transcription Factor II-i, GTF2I, GTFII-I, IB291
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with GTF2I Antibody (C-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).GTF2I immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with GTF2I Antibody (C-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).GTF2I immunoreactivity is localized to Nucleus significantly.)
Western Blot (WB)
(GTF2I Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the GTF2I antibody detected the GTF2I protein (arrow).)
Western Blot (WB)
(GTF2I Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the GTF2I antibody detected the GTF2I protein (arrow).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with GTF2I Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with GTF2I Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(GTF2I Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(GTF2I Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]
Uniprot Description
GTF2I: a multifunctional phosphoprotein with roles in transcription and signal transduction. Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and Phox1 and interacts cooperatively with Phox1 to promote serum-inducible transcription of a reporter gene driven by the C- FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Its gene is known to contain at least 34 exons. Four splice variant isoforms have been described.
Protein type: Transcription, coactivator/corepressor; DNA-binding
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: nucleoplasm; cell projection; membrane; cell soma; cytoplasm; nucleus
Molecular Function: protein binding; DNA binding; mitogen-activated protein kinase binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of angiogenesis; regulation of transcription, DNA-dependent; transition between slow and fast fiber; reduction of cytosolic calcium ion concentration; signal transduction
Disease: Williams-beuren Syndrome
Research Articles on TFII-I
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Product Notes
The TFII-I gtf2i (Catalog #AAA6502405) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TFII-I, CT (Transcription Factor II-I, Bruton Tyrosine Kinase-associated Protein 135, BTK-associated Protein 135, BTK-associated Protein 135kD, BAP135, BAP-135, BTK-associated Protein, BTKAP1, DIWS, General Transcription Factor II-i, GTF2I, GTFII-I, IB291 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TFII-I can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-1:500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TFII-I gtf2i for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFII-I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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