Rabbit SNF8 Polyclonal Antibody | anti-SNF8 antibody

SNF8 Antibody

Cat. Num. AAA9630845

• Gene Names: SNF8; Dot3; EAP30; VPS22
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SNF8; Polyclonal Antibody; SNF8 Antibody; Dot3; ELL associated protein of 30 kDa; ELL-associated protein of 30 kDa; ESCRT-II complex subunit VPS22; hVps22; snf8; SNF8 ESCRTII complex subunit homolog (S cerevisiae); SNF8_HUMAN; Vacuolar sorting protein SNF8; Vacuolar-sorting protein SNF8; VPS22; anti-SNF8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SNF8 Antibody detects endogenous levels of SNF8.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SNF8 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human SNF8.
• Conjugation: Unconjugated
• Similarity: Belongs to the SNF8 family.
• Subunit Structure: Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS25 and VPS36. SNF8 is essential for the stability of the ESCRT-II complex. ESCRT-II interacts with ELL. Interacts with TSG101 (via the C-terminal domain). Interacts with RILPL1 (via the N-terminal domain); which recruits ESCRT-II to the endosome membranes. Interacts with 14-3-3 proteins.
• Function: Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Required for the exosomal release of SDCBP, CD63 and syndecan.
• Note: Recruited to the endosome membrane to participate in vesicle formation.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-SNF8 antibody

Cytoplasm; Endosome membrane; Nucleus; Late endosome membrane; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 21361380
• NCBI GeneID: 11267
• NCBI Accession #: NP_009172.2
• NCBI GenBank Nucleotide #: NM_007241.2
• UniProt Accession #: Q96H20; Q8IXY3; Q9UN50
• Molecular Weight: 28,736 Da
• NCBI Official Full Name: vacuolar-sorting protein SNF8
• NCBI Official Synonym Full Names: SNF8, ESCRT-II complex subunit
• NCBI Official Symbol: SNF8
• NCBI Official Synonym Symbols: Dot3; EAP30; VPS22
• NCBI Protein Information: vacuolar-sorting protein SNF8; EAP30 subunit of ELL complex; ELL-associated protein of 30 kDa; ESCRT-II complex subunit VPS22; SNF8, ESCRT-II complex subunit, homolog; hVps22
• UniProt Protein Name: Vacuolar-sorting protein SNF8
• Protein Family: Vacuolar-sorting protein
• UniProt Gene Name: SNF8
• UniProt Synonym Gene Names: EAP30; hVps22
• UniProt Entry Name: SNF8_HUMAN

NCBI Description

SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM, Mar 2008]

Uniprot Description

SNF8: Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Belongs to the SNF8 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: nucleoplasm; transcription factor complex; late endosome membrane; cytoplasm; cytosol

Molecular Function: protein binding; protein homodimerization activity; transcription factor binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway; endosome transport