Western Blot (WB)
(Western blot analysis of lysate from HT29 cells, using SMYD2 antibody.)
Rabbit SMYD2 Polyclonal Antibody | anti-SMYD2 antibody
SMYD2 Antibody
Gene Names
SMYD2; KMT3C; HSKM-B; ZMYND14
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
SMYD2; Polyclonal Antibody; SMYD2 Antibody; anti-SMYD2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
SMYD2 antibody detects endogenous levels of SMYD2.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
272
Applicable Applications for anti-SMYD2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:10000
ELISA: 1:10000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human SMYD2.
Immunogen Range
99-148
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Western Blot (WB)
(Western blot analysis of lysate from HT29 cells, using SMYD2 antibody.)
Western Blot (WB)
(Western blot analysis of lysate from HT29 cells, using SMYD2 antibody.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49 kDa
NCBI Official Full Name
N-lysine methyltransferase SMYD2
NCBI Official Synonym Full Names
SET and MYND domain containing 2
NCBI Official Symbol
SMYD2
NCBI Official Synonym Symbols
KMT3C; HSKM-B; ZMYND14
NCBI Protein Information
N-lysine methyltransferase SMYD2
UniProt Protein Name
N-lysine methyltransferase SMYD2
Protein Family
UniProt Gene Name
SMYD2
UniProt Synonym Gene Names
KMT3C
NCBI Description
SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM, Nov 2008]
Uniprot Description
Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.
Research Articles on SMYD2
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Product Notes
The SMYD2 smyd2 (Catalog #AAA9509050) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMYD2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SMYD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the SMYD2 smyd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMYD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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