Rabbit anti-Human SMURF1 Polyclonal Antibody | anti-SMURF1 antibody

SMURF1 (E3 Ubiquitin-protein Ligase SMURF1, hSMURF1, SMAD Ubiquitination Regulatory Factor 1, KIAA1625, SMAD-specific E3 Ubiquitin-protein Ligase 1) (FITC)

Cat. Num. AAA6394523

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SMURF1; Polyclonal Antibody; SMURF1 (E3 Ubiquitin-protein Ligase SMURF1; hSMURF1; SMAD Ubiquitination Regulatory Factor 1; KIAA1625; SMAD-specific E3 Ubiquitin-protein Ligase 1) (FITC); EC=6.3.2.-; anti-SMURF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SMURF1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SMURF1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human SMURF1, aa1-731 (NP_851994.1).
• Immunogen Sequence: MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of SMURF1 expression in transfected 293T cell line by SMURF1 polyclonal antibody. Lane 1: SMURF1 transfected lysate (83.4kD). Lane 2: Non-transfected lysate.)

Testing Data

(Proximity Ligation Analysis (PLA) of protein-protein interactions between SMURF1 and ECSIT. HeLa cells were stained with SMURF1 rabbit purified polyclonal 1:1200 and ECSIT mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Product Categories/Family for anti-SMURF1 antibody

Antibodies; Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 31317290
• NCBI GeneID: 57154
• NCBI Accession #: NP_851994.1
• NCBI GenBank Nucleotide #: NM_181349
• UniProt Accession #: Q9HCE7; O75853; Q547Q3; Q9UJT8; A4D279; B7ZMB6; B9EGV3
• Molecular Weight: 83,440 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase SMURF1 isoform 2
• NCBI Official Synonym Full Names: SMAD specific E3 ubiquitin protein ligase 1
• NCBI Official Symbol: SMURF1
• NCBI Protein Information: E3 ubiquitin-protein ligase SMURF1; hSMURF1; E3 ubiquitin ligase SMURF1; Smad-specific E3 ubiquitin ligase 1; Smad ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1
• UniProt Protein Name: E3 ubiquitin-protein ligase SMURF1
• UniProt Gene Name: SMURF1
• UniProt Synonym Gene Names: KIAA1625; hSMURF1
• UniProt Entry Name: SMUF1_HUMAN

NCBI Description

This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Dec 2010]

Uniprot Description

SMURF1: E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Interacts with TRAF4. Interacts (via HECT domain) with FBXL15 (via LRR repeats). Interacts with SMAD7 and TGFBR1; SMAD7 recruits SMURF1 to TGFBR1 and regulates TGF-beta receptor degradation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: axon; cell soma; cytoplasm; cytosol; mitochondrion; nucleoplasm; nucleus; plasma membrane

Molecular Function: activin binding; ligase activity; phospholipid binding; protein binding; ubiquitin-protein ligase activity

Biological Process: BMP signaling pathway; cell differentiation; ectoderm development; negative regulation of BMP signaling pathway; negative regulation of ossification; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of defense response to virus by host; proteasomal ubiquitin-dependent protein catabolic process; protein export from nucleus; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; receptor catabolic process; transforming growth factor beta receptor signaling pathway; ubiquitin-dependent SMAD protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway