Western Blot (WB)
(Western blot analysis of extracts from MCF7, using Phospho-SMURF1(Ser200) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Rabbit SMURF1 Polyclonal Antibody | anti-SMURF1 antibody
Phospho-SMURF1 (Ser200) Antibody
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Tissue Specificity: Expressed in melanocytes.
Peptide ELISA: 1:20,000-1:40,000
Western Blot (WB)
(Western blot analysis of extracts from MCF7, using Phospho-SMURF1(Ser200) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from MCF7, using Phospho-SMURF1(Ser200) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Dec 2010]
Uniprot Description
SMURF1: E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Interacts with TRAF4. Interacts (via HECT domain) with FBXL15 (via LRR repeats). Interacts with SMAD7 and TGFBR1; SMAD7 recruits SMURF1 to TGFBR1 and regulates TGF-beta receptor degradation. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-; Ligase; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: axon; cell soma; cytoplasm; cytosol; mitochondrion; nucleoplasm; nucleus; plasma membrane
Molecular Function: activin binding; ligase activity; phospholipid binding; protein binding; ubiquitin-protein ligase activity
Biological Process: BMP signaling pathway; cell differentiation; ectoderm development; negative regulation of BMP signaling pathway; negative regulation of ossification; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of defense response to virus by host; proteasomal ubiquitin-dependent protein catabolic process; protein export from nucleus; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; receptor catabolic process; transforming growth factor beta receptor signaling pathway; ubiquitin-dependent SMAD protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway
Research Articles on SMURF1
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Product Notes
The SMURF1 smurf1 (Catalog #AAA9613124) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-SMURF1 (Ser200) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's SMURF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the SMURF1 smurf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMURF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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