Western Blot (WB)
(SMPD4 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the SMPD4 antibody detected the SMPD4 protein (arrow).)
Rabbit anti-Human SMPD4 Polyclonal Antibody | anti-SMPD4 antibody
SMPD4, NT (SMPD4, KIAA1418, Sphingomyelin phosphodiesterase 4, Neutral sphingomyelinase 3, Neutral sphingomyelinase III) (Azide free) (HRP)
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Western Blot (WB)
(SMPD4 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the SMPD4 antibody detected the SMPD4 protein (arrow).)
Western Blot (WB)
(SMPD4 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the SMPD4 antibody detected the SMPD4 protein (arrow).)
Immunohistochemistry (IHC)
(SMPD4 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMPD4 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SMPD4 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMPD4 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
Sphingomyelinases (EC 3.1.4.12), such as SMPD4, catalyze the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide (Krut et al., 2006 [PubMed 16517606]).[supplied by OMIM, Mar 2008]
Uniprot Description
SMPD4: Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - sphingolipid; Endoplasmic reticulum; EC 3.1.4.12; Membrane protein, integral; Phosphodiesterase
Chromosomal Location of Human Ortholog: 2q21.1
Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane; trans-Golgi network
Molecular Function: sphingomyelin phosphodiesterase activity; metal ion binding; sphingomyelin phosphodiesterase D activity
Biological Process: sphingolipid metabolic process; glycerophospholipid catabolic process; ceramide biosynthetic process; glycosphingolipid metabolic process; sphingomyelin catabolic process
Research Articles on SMPD4
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Product Notes
The SMPD4 smpd4 (Catalog #AAA6349060) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMPD4, NT (SMPD4, KIAA1418, Sphingomyelin phosphodiesterase 4, Neutral sphingomyelinase 3, Neutral sphingomyelinase III) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMPD4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SMPD4 smpd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMPD4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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