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Western Blot (WB) (Western blot analysis of HIP116A Antibody (Center) in HL60 cell line lysates (35ug/lane). HIP116A (arrow) was detected using the purified Pab.)

Rabbit anti-Human SMARCA3 Polyclonal Antibody | anti-SMARCA3 antibody

SMARCA3 (SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A Member 3, DNA-binding Protein/plasminogen Activator Inhibitor 1 Regulator, Helicase-like Transcription Factor, HLTF, HLTF1, HIP116, HIP116A, RING Finger Protein

Gene Names
HLTF; ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3
Reactivity
Human
Applications
Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
SMARCA3; Polyclonal Antibody; SMARCA3 (SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A Member 3; DNA-binding Protein/plasminogen Activator Inhibitor 1 Regulator; Helicase-like Transcription Factor; HLTF; HLTF1; HIP116; HIP116A; RING Finger Protein; EC=3.6.4.-; EC=6.3.2.-; anti-SMARCA3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human HIP116A.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-SMARCA3 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the center region of human HIP116A (KLH).
Conjugate
MaxLight550
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HIP116A Antibody (Center) in HL60 cell line lysates (35ug/lane). HIP116A (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of HIP116A Antibody (Center) in HL60 cell line lysates (35ug/lane). HIP116A (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(HIP116A Antibody (Center) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (HIP116A Antibody (Center) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-SMARCA3 antibody
References
1. Ding, H., et.al., DNA Cell Biol. 15(6), 429-442 (1996).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
114kDa
NCBI Official Full Name
helicase-like transcription factor isoform 1
NCBI Official Synonym Full Names
helicase like transcription factor
NCBI Official Symbol
HLTF
NCBI Official Synonym Symbols
ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3
NCBI Protein Information
helicase-like transcription factor
UniProt Protein Name
Helicase-like transcription factor
UniProt Gene Name
HLTF
UniProt Synonym Gene Names
HIP116A; RNF80; SMARCA3; SNF2L3; ZBU1

NCBI Description

This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SMARCA3: an enzyme with apparent ATP-dependent DNA helicase activity. Belongs to the SNF2/RAD54 helicase family, RAD16 subfamily. Two alternatively spliced human isoforms have been described.

Protein type: DNA-binding; EC 3.6.1.-; EC 3.6.4.-; EC 6.3.2.-; EC 6.3.2.19; Helicase; Ligase; Nucleolus; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: membrane; nucleoplasm; nucleus

Molecular Function: DNA binding; protein binding; ubiquitin protein ligase binding

Biological Process: regulation of transcription, DNA-dependent

Research Articles on SMARCA3

Similar Products

Product Notes

The SMARCA3 hltf (Catalog #AAA6499871) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMARCA3 (SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A Member 3, DNA-binding Protein/plasminogen Activator Inhibitor 1 Regulator, Helicase-like Transcription Factor, HLTF, HLTF1, HIP116, HIP116A, RING Finger Protein reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMARCA3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SMARCA3 hltf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMARCA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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