Rabbit anti-Human Small Heterodimer Partner Polyclonal Antibody | anti-SHP antibody

Small Heterodimer Partner (SHP) Polyclonal Antibody

Cat. Num. AAA2032485

• Gene Names: NR0B2; SHP; SHP1
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Small Heterodimer Partner; Polyclonal Antibody; Small Heterodimer Partner (SHP) Polyclonal Antibody; NR0B2; SHP1; Nuclear Receptor Subfamily 0; Group B; Member 2; Orphan nuclear receptor SHP; anti-SHP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against SHP. It has been selected for its ability to recognize SHP in immunohistochemical staining and western blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 257

• Applicable Applications for anti-SHP antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western Blot: 1:50-400; Immunohistochemistry in formalin fixed frozen section: 1:50-500; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunoglobulin Type: IgG
• Conjugate: No Conjugate
• Cross Reactivity: Human, Pig
• Organism Species: Human
• Fragment: SHP (Met1~Arg257)
• Quality Control: Content: The quality control contains recombinant SHP (Met1~Arg257) disposed in loading buffer.; Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.; 5uL per well when used in enhanced chemilumescent (ECL).; Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.; Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2064407
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Porcine Kidney lysate; Primary Ab: 1ug/ml Rabbit Anti-Human SHP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Human Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 13259503
• NCBI GeneID: 8431
• NCBI Accession #: NP_068804.1
• NCBI GenBank Nucleotide #: NM_021969.2
• UniProt Accession #: Q15466; Q5QP36; F1D8P5
• Molecular Weight: 28,058 Da
• NCBI Official Full Name: nuclear receptor subfamily 0 group B member 2
• NCBI Official Synonym Full Names: nuclear receptor subfamily 0 group B member 2
• NCBI Official Symbol: NR0B2
• NCBI Official Synonym Symbols: SHP; SHP1
• NCBI Protein Information: nuclear receptor subfamily 0 group B member 2
• UniProt Protein Name: Nuclear receptor subfamily 0 group B member 2
• Protein Family: Head decoration protein
• UniProt Gene Name: NR0B2
• UniProt Synonym Gene Names: SHP

NCBI Description

The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. [provided by RefSeq, Jul 2008]

Uniprot Description

NR0B2: Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box- containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1. Defects in NR0B2 may be associated with obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the nuclear hormone receptor family. NR0 subfamily.

Protein type: Nuclear receptor; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: cytoplasm; nucleoplasm; nucleus; protein complex

Molecular Function: DNA binding; peroxisome proliferator activated receptor binding; protein binding; protein complex binding; protein domain specific binding; protein homodimerization activity; retinoid X receptor binding; steroid hormone receptor activity; thyroid hormone receptor binding; transcription corepressor activity

Biological Process: cholesterol metabolic process; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; Notch signaling pathway; organ regeneration; positive regulation of insulin secretion; response to glucose stimulus; steroid hormone mediated signaling; transcription initiation from RNA polymerase II promoter

Disease: Obesity