Rabbit anti-Human SMAD6 Polyclonal Antibody | anti-SMAD6 antibody

SMAD6, ID (SMAD6, MADH6, Mothers against decapentaplegic homolog 6, SMAD family member 6) (PE)

Cat. Num. AAA6348791

• Gene Names: SMAD6; AOVD2; MADH6; MADH7; HsT17432
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SMAD6; Polyclonal Antibody; ID (SMAD6; MADH6; Mothers against decapentaplegic homolog 6; SMAD family member 6) (PE); SMAD family member 6; anti-SMAD6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SMAD6 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SMAD6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-396 amino acids from the Central region of human SMAD6.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SMAD6 Antibody (Center) western blot analysis in HepG2,HT29 cell line lysates (35ug/lane).This demonstrates the SMAD6 antibody detected the SMAD6 protein (arrow).)

Flow Cytometry (FC/FACS)

(SMAD6 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SMAD6 antibody

SMAD6 belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling.

Product Categories/Family for anti-SMAD6 antibody

Antibodies; Smad Proteins

References

Tseng, Z.H., et al. Heart Rhythm 6(12):1745-1750(2009). Shintani, M., et al. J. Med. Genet. 46(5):331-337(2009). Yu, H., et al. Acta Derm. Venereol. 89(4):351-356(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 92859871
• NCBI GeneID: 4091
• NCBI Accession #: NP_005576.3
• NCBI GenBank Nucleotide #: NM_005585.4
• UniProt Accession #: O43541; O43654; Q15799; Q7Z7L4; Q96E31; Q9UKZ3; A9J6M5
• Molecular Weight: 35,467 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 6
• NCBI Official Synonym Full Names: SMAD family member 6
• NCBI Official Symbol: SMAD6
• NCBI Official Synonym Symbols: AOVD2; MADH6; MADH7; HsT17432
• NCBI Protein Information: mothers against decapentaplegic homolog 6
• UniProt Protein Name: Mothers against decapentaplegic homolog 6
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: SMAD6
• UniProt Synonym Gene Names: MADH6; MAD homolog 6; Mothers against DPP homolog 6; SMAD 6; Smad6; hSMAD6
• UniProt Entry Name: SMAD6_HUMAN

NCBI Description

The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014]

Uniprot Description

SMAD6: Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions. Interacts with NEDD4L. Interacts with WWP1. Interacts with RNF111 and AXIN1. Interacts with TGF-beta type I receptor superfamily members, SMAD1, HOXC8 and HOXC9. Interacts with STAMBP and PRKX. Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue. Belongs to the dwarfin/SMAD family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: transcription factor complex; protein complex; cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; ubiquitin protein ligase binding; metal ion binding; chromatin binding; transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity; transcription factor activity

Biological Process: BMP signaling pathway; fat cell differentiation; negative regulation of cell proliferation; zygotic determination of dorsal/ventral axis; transcription, DNA-dependent; ureteric bud development; response to estrogen stimulus; transforming growth factor beta receptor signaling pathway; immune response; negative regulation of transforming growth factor beta receptor signaling pathway; cell-substrate adhesion; negative regulation of apoptosis; negative regulation of BMP signaling pathway

Disease: Aortic Valve Disease 2