Rabbit anti-Human SMAD4 Polyclonal Antibody | anti-SMAD4 antibody

SMAD4, CT (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP Homolog 4, Deletion Target In Pancreatic Carcinoma 4, MAD Homolog 4, SMAD Family Member 4, hSMAD4, DPC4, MADH4) (MaxLight 405)

Cat. Num. AAA6499858

• Gene Names: SMAD4; JIP; DPC4; MADH4; MYHRS
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: SMAD4; Polyclonal Antibody; CT (Mothers Against Decapentaplegic Homolog 4; SMAD 4; Mothers Against DPP Homolog 4; Deletion Target In Pancreatic Carcinoma 4; MAD Homolog 4; SMAD Family Member 4; hSMAD4; DPC4; MADH4) (MaxLight 405); anti-SMAD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SMAD4.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-SMAD4 antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-1:50; FLISA: 1:1,000; IF: 1:10-1:50; IHC: 1:50-1:100; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human SMAD4 (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SMAD4 (arrow) using rabbit polyclonal SMAD4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with SMAD4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cell stained with SMAD4 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD4 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SMAD4 immunoreactivity is localized to Cytoplasm and Nucleus significantly. )

Flow Cytometry (FC/FACS)

(SMAD4 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SMAD4 antibody

Antibodies; Smad Proteins

References

1. Sekiya, T., et al., Biochem. Biophys. Res. Commun. 320(3):680-684 (2004). 2. Horvath, L.G., et al., Prostate 59(3):234-242 (2004). 3. Li, L., et al., Mol. Cell. Biol. 24(2):856-864 (2004). 4. Wan, M., et al., J. Biol. Chem. 279(15):14484-14487 (2004). 5. Maru, D., et al., Oncogene 23(3):859-864 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4885457
• NCBI GeneID: 4089
• NCBI Accession #: NP_005350
• NCBI GenBank Nucleotide #: NM_005359.5
• UniProt Accession #: Q13485; A8K405
• Molecular Weight: 62.6 kDa (572 aa)
• NCBI Official Full Name: mothers against decapentaplegic homolog 4
• NCBI Official Synonym Full Names: SMAD family member 4
• NCBI Official Symbol: SMAD4
• NCBI Official Synonym Symbols: JIP; DPC4; MADH4; MYHRS
• NCBI Protein Information: mothers against decapentaplegic homolog 4
• UniProt Protein Name: Mothers against decapentaplegic homolog 4
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: SMAD4
• UniProt Synonym Gene Names: DPC4; MADH4; MAD homolog 4; Mothers against DPP homolog 4; SMAD 4; Smad4; hSMAD4
• UniProt Entry Name: SMAD4_HUMAN

NCBI Description

This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasng blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Aug 2017]

Uniprot Description

SMAD4: transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: nucleoplasm; centrosome; transcription factor complex; cytoplasm; nuclear chromatin; nucleus; cytosol

Molecular Function: collagen binding; identical protein binding; protein binding; protein homodimerization activity; transforming growth factor beta receptor, common-partner cytoplasmic mediator activity; DNA binding; sequence-specific DNA binding; metal ion binding; chromatin binding; transcription factor activity

Biological Process: developmental growth; axon guidance; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; sebaceous gland development; negative regulation of transcription from RNA polymerase II promoter; palate development; BMP signaling pathway; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; mesoderm development; neural crest cell differentiation; positive regulation of BMP signaling pathway; transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor-beta2 production; transcription, DNA-dependent; regulation of binding; in utero embryonic development; neuron fate commitment; positive regulation of transforming growth factor beta receptor signaling pathway; gastrulation with mouth forming second; somite rostral/caudal axis specification; formation of anatomical boundary; SMAD protein complex assembly; endothelial cell activation; cell proliferation; ureteric bud branching; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; gene expression; regulation of hair follicle development; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; negative regulation of protein catabolic process; endoderm development

Disease: Pancreatic Cancer; Juvenile Polyposis Syndrome; Myhre Syndrome; Juvenile Polyposis/hereditary Hemorrhagic Telangiectasia Syndrome