Rabbit SMAD3 Polyclonal Antibody | anti-SMAD3 antibody

RABBIT ANTI SMAD3

Cat. Num. AAA222915

• Gene Names: SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
• Reactivity: Amphibia, Bovine, Chicken, Mouse, Pig, Rat, Zebrafish
• Applications: ELISA, Immunohistochemistry, Western Blot
• Synonyms: SMAD3; Polyclonal Antibody; RABBIT ANTI SMAD3; anti-SMAD3 antibody

• Host: Rabbit
• Reactivity: Amphibia, Bovine, Chicken, Mouse, Pig, Rat, Zebrafish
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises Mothers against decapentaplegic homolog 3 (SMAD3), a 50kDa transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase, ; ; SMAD3 functions in cell proliferation, differentiation and development. Upon stimulation, SMAD3 oligomerizes with SMAD4 and translocates into the nucleus where it binds to DNA and transcription factors. SMAD3 activity induces differentiation of selected neuronal subtypes at the expense of other subtypes. ; ; Studies have indicated that defects in SMAD3 may be a cause of colorectal cancer.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 320

• Applicable Applications for anti-SMAD3 antibody: ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: ELISA: Minimum Dilution: 1/25000; Maximum Dilution: 1/100000;; Immunohistology - Paraffin: Maximum Dilution: 2.5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 1/2000; Maximum Dilution: 1/20000
• Perservative Stabilisers: 0.01% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide corresponding to amino acids 417-425 of human SMAD3 protein.; Histology Positive Control Tissue
• Antiserum Preparation: Antiserum to human SMAD3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human skin with Rabbit anti Mouse SMAD3 (MBS222915))

Related Product Information for anti-SMAD3 antibody

MBS222915 recognises Mothers against decapentaplegic homolog 3 (SMAD3), a 50kDa transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase, SMAD3 functions in cell proliferation, differentiation and development. Upon stimulation, SMAD3 oligomerizes with SMAD4 and translocates into the nucleus where it binds to DNA and transcription factors. SMAD3 activity induces differentiation of selected neuronal subtypes at the expense of other subtypes. Studies have indicated that defects in SMAD3 may be a cause of colorectal cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 223029440
• NCBI GeneID: 4088
• NCBI Accession #: NP_001138574.1
• NCBI GenBank Nucleotide #: NM_001145102.1
• UniProt Accession #: P84022; O09064; O09144; O14510; O35273; Q92940; Q93002; A8K4B6; B7Z4Z5; B7Z6M9; B7Z9Q2; F5H383
• Molecular Weight: 25,722 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 3 isoform 2
• NCBI Official Synonym Full Names: SMAD family member 3
• NCBI Official Symbol: SMAD3
• NCBI Official Synonym Symbols: LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
• NCBI Protein Information: mothers against decapentaplegic homolog 3; MAD homolog 3; MAD, mothers against decapentaplegic homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; hMAD-3; hSMAD3; mad homolog JV15-2; mad protein homolog; mad3
• UniProt Protein Name: Mothers against decapentaplegic homolog 3
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: SMAD3
• UniProt Synonym Gene Names: MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3
• UniProt Entry Name: SMAD3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

Uniprot Description

SMAD3: transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

Protein type: Nuclear receptor co-regulator; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 15q22.33

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nuclear chromatin; plasma membrane; nuclear inner membrane; cytosol; nucleus; receptor complex

Molecular Function: identical protein binding; ubiquitin binding; protein homodimerization activity; zinc ion binding; chromatin DNA binding; beta-catenin binding; transcription factor binding; protein kinase binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; phosphatase binding; collagen binding; protein binding; sequence-specific DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; bHLH transcription factor binding; transforming growth factor beta receptor binding; transcription factor activity

Biological Process: developmental growth; positive regulation of positive chemotaxis; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; embryonic pattern specification; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; embryonic foregut morphogenesis; positive regulation of stress fiber formation; negative regulation of osteoblast differentiation; negative regulation of mitotic cell cycle; cell cycle arrest; regulation of striated muscle development; pericardium development; somitogenesis; transcription, DNA-dependent; embryonic cranial skeleton morphogenesis; osteoblast development; regulation of transcription from RNA polymerase II promoter; positive regulation of chondrocyte differentiation; mesoderm formation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of osteoblast proliferation; negative regulation of protein catabolic process; negative regulation of apoptosis; endoderm development; evasion of host defenses by virus; T cell activation; wound healing; negative regulation of transcription from RNA polymerase II promoter; primary microRNA processing; regulation of epithelial cell proliferation; positive regulation of focal adhesion formation; negative regulation of protein amino acid phosphorylation; transport; ureteric bud development; positive regulation of transforming growth factor-beta3 production; positive regulation of transcription factor import into nucleus; thyroid gland development; heart looping; caspase activation; transcription initiation from RNA polymerase II promoter; intercellular junction assembly and maintenance; regulation of immune response; regulation of transforming growth factor-beta2 production; regulation of binding; protein stabilization; in utero embryonic development; liver development; positive regulation of bone mineralization; SMAD protein complex assembly; immune system development; positive regulation of interleukin-1 beta production; negative regulation of inflammatory response; response to hypoxia; gene expression; immune response; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration

Disease: Loeys-dietz Syndrome 3