Western Blot (WB)
(Western blot analysis of extract from Hela cells untreated or treated with IL-4 (100ng/mL, 30min), using Smad2 antibody and Smad2 antibody (Thr220) (Lanes 3 and 4))
Rabbit SMAD2 Polyclonal Antibody | anti-SMAD2 antibody
SMAD2 Antibody (Thr220)
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
SMAD2; Polyclonal Antibody; SMAD2 Antibody (Thr220); Rabbit Polyclonal SMAD2 Antibody (Thr220); Polyclonal Smad2 antibody; Anti-Smad2 antibody; Mothers against decapentaplegic homolog 2 antibody; Mothers against DPP homolog 2 antibody; Sma- and Mad-related protein 2 antibody; SMAD 2 antibody; SMAD family member 2 antibody; SMAD mothers against DPP homolog 2 antibody; SMAD2 antibody; hMAD-2 antibody; hSMAD2 antibody; JV18 antibody; JV18-1 antibody; MAD homolog 2 antibody; Mad protein homolog antibody; MAD mothers against decapentaplegic homolog 2 antibody; Mad-related protein 2 antibody; MADH2 antibody; MADR2 antibody; MGC22139 antibody; MGC34440 antibody; mother against DPP homolog 2 antibody; anti-SMAD2 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Smad2 antibody (Thr220) detects endogenous levels of Smad2 only when phosphorylated at threonine 220
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
467
Applicable Applications for anti-SMAD2 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Smad2 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human Smad2 around the phosphorylation site of threonine 220 (P-E-TP-P-P) as the immunogen.
Cross-Reactivity
Mouse, Rat
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extract from Hela cells untreated or treated with IL-4 (100ng/mL, 30min), using Smad2 antibody and Smad2 antibody (Thr220) (Lanes 3 and 4))
Western Blot (WB)
(Western blot analysis of extract from Hela cells untreated or treated with IL-4 (100ng/mL, 30min), using Smad2 antibody and Smad2 antibody (Thr220) (Lanes 3 and 4))
Related Product Information for anti-SMAD2 antibody
Smad2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.
Product Categories/Family for anti-SMAD2 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The SMAD2 (Catalog #AAA5313724) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SMAD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the SMAD2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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