Western Blot (WB)
(Western blot analysis of Smac in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate with Smac antibody at 1 μg /ml. )
Rabbit Smac Polyclonal Antibody | anti-Diablo antibody
Smac Antibody
Western Blot (WB)
(Western blot analysis of Smac in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate with Smac antibody at 1 μg /ml. )
Western Blot (WB)
(Western blot analysis of Smac in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate with Smac antibody at 1 μg /ml. )
Immunohistochemistry (IHC)
(Immunohistochemical staining of mouse spleen tissue using Smac antibody at 2 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of mouse spleen tissue using Smac antibody at 2 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of Smac in Mouse Spleen cells with Smac antibody at 10 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of Smac in Mouse Spleen cells with Smac antibody at 10 μg/mL.)
NCBI and Uniprot Product Information
Uniprot Description
DIABLO: Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. Homodimer. Interacts with NGFRAP1/BEX3. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin. Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis; Mitochondrial
Cellular Component: internal side of plasma membrane; mitochondrion; cytoplasm; mitochondrial intermembrane space
Biological Process: caspase activation; neuron apoptosis; positive regulation of apoptosis; apoptosis; caspase activation via cytochrome c; induction of apoptosis by oxidative stress
Research Articles on Diablo
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Product Notes
The Diablo diablo (Catalog #AAA150865) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Smac Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Smac can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). Smac antibody can be used for detection of Smac by Western blot at 1 mug/mL. A 25 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 10 mug/mL. Researchers should empirically determine the suitability of the Diablo diablo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smac, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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