Western Blot (WB)
((0. 02ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SM22 Alpha/Transgelin Polyclonal Antibody | anti-TAGLN antibody
Goat anti-SM22 Alpha/Transgelin Antibody
Gene Names
TAGLN; SM22; SMCC; TAGLN1; WS3-10
Reactivity
Tested: Human, Mouse, Rat, Rabbit; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
SM22 Alpha/Transgelin; Polyclonal Antibody; Goat anti-SM22 Alpha/Transgelin Antibody; TAGLN; SM22; SMCC; WS3-10; transgelin; SM22-alpha; DKFZp686P11128; TAGLN1; DKFZp686B01212; transgelin variant 2; TAGLN antibody; SM22 antibody; SMCC antibody; WS3-10 antibody; transgelin antibody; SM22-alpha antibody; DKFZp686P11128 antibody; TAGLN1 antibody; DKFZp686B01212 antibody; transgelin variant 2 antibody; Transgelin; SM22 Alpha / Transgelin; anti-TAGLN antibody
Host
Goat
Reactivity
Tested: Human, Mouse, Rat, Rabbit; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
MTGYGRPRQIIS
Sequence Length
201
Applicable Applications for anti-TAGLN antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 24kDa band observed in Human Placenta lysates (calculated MW of 22.6kDa according to NP_003177.2). Recommended concentration: 0.01-0.03ug/ml.
Western Blot: Approx 24kDa band observed in Human Placenta lysates (calculated MW of 22.6kDa according to NP_003177.2). Recommended concentration: 0.01-0.03ug/ml.
Immunogen
Peptide with sequence C-MTGYGRPRQIIS, from the C Terminus of the protein sequence according to NP_003177.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0. 02ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 02ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,611 Da
NCBI Official Full Name
transgelin
NCBI Official Synonym Full Names
transgelin
NCBI Official Symbol
TAGLN
NCBI Official Synonym Symbols
SM22; SMCC; TAGLN1; WS3-10
NCBI Protein Information
transgelin
UniProt Protein Name
Transgelin
Protein Family
UniProt Gene Name
TAGLN
UniProt Synonym Gene Names
SM22; WS3-10; SM22-alpha
UniProt Entry Name
TAGL_HUMAN
NCBI Description
The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
TAGLN: Actin cross-linking/gelling protein. Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. Belongs to the calponin family.
Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Actin-binding
Chromosomal Location of Human Ortholog: 11q23.2
Cellular Component: cytoplasm
Molecular Function: actin binding; protein binding
Biological Process: epithelial cell differentiation; muscle development
Research Articles on TAGLN
Similar Products
Product Notes
The TAGLN tagln (Catalog #AAA420744) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SM22 Alpha/Transgelin Antibody reacts with Tested: Human, Mouse, Rat, Rabbit; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's SM22 Alpha/Transgelin can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 24kDa band observed in Human Placenta lysates (calculated MW of 22.6kDa according to NP_003177.2). Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the TAGLN tagln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MTGYGRPRQI IS. It is sometimes possible for the material contained within the vial of "SM22 Alpha/Transgelin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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