Chicken Cystine/glutamate transporter (SLC7A11) ELISA Kit | SLC7A11 elisa kit

Chicken Cystine/glutamate transporter (SLC7A11) ELISA Kit

Cat. Num. AAA7232478

• Gene Names: SLC7A11; xCT; CCBR1
• Reactivity: Chicken
• Synonyms: Cystine/glutamate transporter (SLC7A11); Chicken Cystine/glutamate transporter (SLC7A11) ELISA Kit; SLC7A11 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This SLC7A11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken SLC7A11. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLC7A11 elisa kit

Priciple of the assay: SLC7A11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SLC7A11 antibody and an SLC7A11-HRP conjugate. The assay sample and buffer are incubated together with SLC7A11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC7A11 concentration since SLC7A11 from samples and SLC7A11-HRP conjugate compete for the anti-SLC7A11 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC7A11 from the sample, fewer sites are left to bind SLC7A11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC7A11 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7657683
• NCBI GeneID: 23657
• NCBI Accession #: NP_055146.1
• NCBI GenBank Nucleotide #: NM_014331.3
• Molecular Weight: 55,423 Da
• NCBI Official Full Name: cystine/glutamate transporter
• NCBI Official Synonym Full Names: solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11
• NCBI Official Symbol: SLC7A11
• NCBI Official Synonym Symbols: xCT; CCBR1
• NCBI Protein Information: cystine/glutamate transporter; amino acid transport system xc-; solute carrier family 7 member 11; calcium channel blocker resistance protein CCBR1; solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
• UniProt Protein Name: Cystine/glutamate transporter
• Protein Family: Cystine/glutamate transporter
• UniProt Gene Name: SLC7A11
• UniProt Entry Name: XCT_HUMAN

NCBI Description

This gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. This protein has been identified as the predominant mediator of Kaposi sarcoma-associated herpesvirus fusion and entry permissiveness into cells. Also, increased expression of this gene in primary gliomas (compared to normal brain tissue) was associated with increased glutamate secretion via the XCT channels, resulting in neuronal cell death. [provided by RefSeq, Sep 2011]

Uniprot Description

SLC7A11: Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate. Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

Protein type: Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 4q28.3

Cellular Component: cell surface; cytoskeleton; rough endoplasmic reticulum; integral to membrane; plasma membrane

Molecular Function: cystine:glutamate antiporter activity; protein binding

Biological Process: response to nicotine; amino acid transport; response to toxin; ion transport; brain development; response to oxidative stress; blood coagulation; leukocyte migration; transmembrane transport