Typical Testing Data/Standard Curve (for reference only)
Human Follicle Stimulating Hormone (FSH) ELISA Kit | FSH elisa kit
Human Follicle Stimulating Hormone (FSH) ELISA Kit
Gene Names
FSHB; HH24
Reactivity
Human
Synonyms
Follicle Stimulating Hormone (FSH); Human Follicle Stimulating Hormone (FSH) ELISA Kit; FSH elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of FSH. No significant cross-reactivity or interference between FSH and analogues was observed.
Sequence Length
129
Samples
Gallus Serum, Plasma And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
15.6-1,000pg/mL
Sensitivity
6.3pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FSH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FSH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FSH elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FSH in gallus serum, plasma and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FSH. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FSH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FSH. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FSH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
14,700 Da
NCBI Official Full Name
follicle-stimulating hormone
NCBI Official Synonym Full Names
follicle stimulating hormone beta subunit
NCBI Official Symbol
FSHB
NCBI Official Synonym Symbols
HH24
NCBI Protein Information
follitropin subunit beta
UniProt Protein Name
Follitropin subunit beta
UniProt Gene Name
FSHB
UniProt Synonym Gene Names
FSH-B; FSH-beta
NCBI Description
The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Uniprot Description
Stimulates development of follicle and spermatogenesis in the reproductive organs.
Research Articles on FSH
Similar Products
Product Notes
The Human FSH fshb (Catalog #AAA2702554) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2702554 ELISA Kit recognizes Human FSH. It is sometimes possible for the material contained within the vial of "Follicle Stimulating Hormone (FSH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
