Rabbit SLC2A4 / GLUT4 / GLUT-4 Polyclonal Antibody | anti-SLC2A4 antibody

Rabbit Polyclonal to Human SLC2A4 / GLUT4 / GLUT-4

Cat. Num. AAA240694

• Gene Names: SLC2A4; GLUT4
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation
• Purity: Affinity Purified
• Synonyms: SLC2A4 / GLUT4 / GLUT-4; Polyclonal Antibody; Rabbit Polyclonal to Human SLC2A4 / GLUT4 / GLUT-4; Anti-SLC2A4 / GLUT4 / GLUT-4 Antibody IHC-plus; SLC2A4; Glucose transporter 4; Glucose transporter GLUT4; GLUT4; GLUT-4; Human SLC2A4; anti-SLC2A4 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes glucose transporter 4 (GLUT4). It does not react with HEP G2 type glucose transporter in human erythrocytes, rat brain or other tissues that do not exhibit sensitivity to insulin.
• Purity/Purification: Affinity Purified
• Form/Format: PBS, 0.09% sodium azide.
• Concentration: 5 mg/ml (varies by lot)
• Sequence Length: 509

• Applicable Applications for anti-SLC2A4 antibody: Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: IHC-Fr, IHC-P (10 - 20 ug/ml), IP, WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibo...
• Target Species: Human
• Non Reactive Species: Pig
• Immunogen Description: A 12 amino acid peptide corresponding to the carboxyl terminus of the insulin regulatable glucose transporter conjugated to keyhole limpet hemocyanin.
• Immunogen Type: Synthetic peptide - KLH conjugated
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-SLC2A4 / GLUT4 antibody IHC of human heart, cardiac myocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

Related Product Information for anti-SLC2A4 antibody

Glucose Transporter 4 (GLUT4/SLC2A4) is a member of a family of proteins that facilitate glucose transport. In response to insulin GLUT4 translocates from low density microsomes to the plasma membrane. Activation of phosphatidylinositol-3-OH kinase is required for this trafficking event but is not sufficient.

Product Categories/Family for anti-SLC2A4 antibody

Family: Transporter; Subfamily: Sugar transporter

NCBI and Uniprot Product Information

• NCBI GI #: 4507011
• NCBI GeneID: 6517
• NCBI Accession #: NP_001033.1
• NCBI GenBank Nucleotide #: NM_001042.2
• UniProt Accession #: P14672; Q05BQ3; Q14CX2
• Molecular Weight: 43,760 Da
• NCBI Official Full Name: solute carrier family 2, facilitated glucose transporter member 4
• NCBI Official Synonym Full Names: solute carrier family 2 (facilitated glucose transporter), member 4
• NCBI Official Symbol: SLC2A4
• NCBI Official Synonym Symbols: GLUT4
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 4; GLUT-4; insulin-responsive glucose transporter type 4; glucose transporter type 4, insulin-responsive
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 4
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC2A4
• UniProt Synonym Gene Names: GLUT4; GLUT-4
• UniProt Entry Name: GTR4_HUMAN

NCBI Description

This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008]

Uniprot Description

GLUT4: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. Its surface expression is regulated by insulin. Has two internalization sequences, a dileucine repeat present in the C-terminus and a FxxY motif in the amino-terminal end. Associates with an intracellular tubulo-vesicular compartment under low plasma conditions. The binding of insulin to the insulin receptor (a typosine kinase) leads to the rapid translocation of GLUT4 to the cell surface, increasing cellular glucose transport activity. Its translocation to the plasma membrane is also stimulated by exercise, but independent from insulin signaling. This insulin-independent pathway may be regulated by AMPK.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: multivesicular body; trans-Golgi network transport vesicle; clathrin-coated vesicle; membrane; perinuclear region of cytoplasm; integral to plasma membrane; T-tubule; plasma membrane; endomembrane system; coated pit; vesicle membrane; cytosol; external side of plasma membrane; lipid raft

Molecular Function: D-glucose transmembrane transporter activity; protein binding; glucose transmembrane transporter activity

Biological Process: amylopectin biosynthetic process; response to ethanol; cellular response to insulin stimulus; glucose import; hexose transport; carbohydrate metabolic process; brown fat cell differentiation; pathogenesis; glucose transport; glucose homeostasis; transmembrane transport

Disease: Diabetes Mellitus, Noninsulin-dependent