Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using SLC27A5 antibody.)
Rabbit anti-Human SLC27A5 Polyclonal Antibody | anti-SLC27A5 antibody
SLC27A5 Antibody
IHC: 1:50-1:100
IF: 1:100-1:500
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using SLC27A5 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using SLC27A5 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using SLC27A5 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using SLC27A5 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using SLC27A5 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of A549 cells, using SLC27A5 antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acids containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid elongation or complex lipid synthesis rather than in degradation. This gene has a mouse ortholog. [provided by RefSeq, Jul 2008]
Uniprot Description
SLC27A5: Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7- alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Belongs to the ATP-dependent AMP-binding enzyme family.
Protein type: Ligase; Membrane protein, integral; Lipid Metabolism - primary bile acid biosynthesis; Endoplasmic reticulum; EC 6.2.1.7; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 19q13.43
Cellular Component: endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; basal plasma membrane; integral to endoplasmic reticulum membrane
Molecular Function: cholate-CoA ligase activity; fatty acid transporter activity; protein complex binding; very-long-chain-fatty-acid-CoA ligase activity; ATP binding; long-chain-fatty-acid-CoA ligase activity
Biological Process: bile acid and bile salt transport; bile acid biosynthetic process; bile acid metabolic process; very-long-chain fatty acid metabolic process; ketone body biosynthetic process; long-chain fatty acid metabolic process; triacylglycerol mobilization; plasma membrane long-chain fatty acid transport
Research Articles on SLC27A5
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Product Notes
The SLC27A5 slc27a5 (Catalog #AAA7132813) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLC27A5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SLC27A5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:3000 IHC: 1:50-1:100 IF: 1:100-1:500. Researchers should empirically determine the suitability of the SLC27A5 slc27a5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC27A5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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