Methylenetetrahydrofolate Reductase Recombinant Protein | MTHFR recombinant protein

Recombinant Methylenetetrahydrofolate Reductase (MTHFR)

Cat. Num. AAA2030985

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Methylenetetrahydrofolate Reductase; Recombinant Methylenetetrahydrofolate Reductase (MTHFR); MTHFR recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MVNEARGNSS LNPCLEGSAS SGSESSKDSS RCSTPGLDPE RHERLREKMR RRLESGDKWF SLEFFPPRTA EGAVNLISRF DRMAAGGPLY IDVTWHPAGD PGSDKETSSM MIASTAVNYC GLETILHMTC CRQRLEEITG HLHKAKQLGL KNIMALRGDP IGDQWEEEEG GFNYAVDLVK HIRSEFGDYF DICVAGYPKG HPEAGSFEAD LKHLKEKVSA GADFIITQLF FEADTFFRFV KACTDMGITC PIVPGIFPIQ GYHSLRQLVK LSKLEVPQEI KDVIEPIKDNDAAIRNYGIE LAVSLCQELL ASGLVPGLHF YTLNR
• Sequence Length: 656

• Applicable Applications for MTHFR recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 39.8kDa
• Accurate Molecular Mass (KD): 40kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Arg325
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 87240000
• NCBI GeneID: 4524
• NCBI Accession #: NP_005948.3
• NCBI GenBank Nucleotide #: NM_005957.4
• Molecular Weight: 78,965 Da
• NCBI Official Full Name: methylenetetrahydrofolate reductase
• NCBI Official Synonym Full Names: methylenetetrahydrofolate reductase (NAD(P)H)
• NCBI Official Symbol: MTHFR
• NCBI Protein Information: methylenetetrahydrofolate reductase
• UniProt Protein Name: Methylenetetrahydrofolate reductase
• Protein Family: Methylenetetrahydrofolate reductase
• UniProt Gene Name: MTHFR
• UniProt Entry Name: MTHR_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.[provided by RefSeq, Oct 2009]

Uniprot Description

MTHFR: Catalyzes the conversion of 5,10- methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co- substrate for homocysteine remethylation to methionine. Defects in MTHFR are the cause of methylenetetrahydrofolate reductase deficiency (MTHFRD). MTHFRD is autosomal recessive disorder with a wide range of features including homocysteinuria, homocysteinemia, developmental delay, severe mental retardation, perinatal death, psychiatric disturbances, and later-onset neurodegenerative disorders. Defects in MTHFR may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in MTHFR may be a cause of susceptibility to folate-sensitive neural tube defects (FS-NTD). The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Belongs to the methylenetetrahydrofolate reductase family.

Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 1.5.1.20; Energy Metabolism - methane

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: neuron projection; cytosol

Molecular Function: FAD binding; methylenetetrahydrofolate reductase (NADPH) activity; NADP binding

Biological Process: amino acid metabolic process; response to drug; vitamin metabolic process; methionine biosynthetic process; blood circulation; S-adenosylmethionine metabolic process; homocysteine metabolic process; one-carbon compound metabolic process; response to folic acid; response to vitamin B2; response to hypoxia; folic acid metabolic process; water-soluble vitamin metabolic process

Disease: Schizophrenia; Thrombophilia Due To Thrombin Defect; Neural Tube Defects, Folate-sensitive; Homocystinuria Due To Deficiency Of N(5,10)-methylenetetrahydrofolate Reductase Activity