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Western Blot (WB) (SLC25A31 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC25A31 antibody detected the SLC25A31 protein (arrow).)

Rabbit anti-Human SLC25A31 Polyclonal Antibody | anti-SLC25A31 antibody

SLC25A31, ID (SLC25A31, AAC4, ANT4, SFEC, ADP/ATP translocase 4, ADP,ATP carrier protein 4, Adenine nucleotide translocator 4, Solute carrier family 25 member 31, Sperm flagellar energy carrier protein) (Biotin)

Gene Names
SLC25A31; AAC4; ANT4; SFEC35kDa
Reactivity
Human
Applications
Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SLC25A31; Polyclonal Antibody; ID (SLC25A31; AAC4; ANT4; SFEC; ADP/ATP translocase 4; ADP; ATP carrier protein 4; Adenine nucleotide translocator 4; Solute carrier family 25 member 31; Sperm flagellar energy carrier protein) (Biotin); Sperm flagellar energy carrier protein; anti-SLC25A31 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-SLC25A31 antibody
Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:10-50
ELISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
SLC25A31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-175 amino acids from the Central region of human SLC25A31.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SLC25A31 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC25A31 antibody detected the SLC25A31 protein (arrow).)

Western Blot (WB) (SLC25A31 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC25A31 antibody detected the SLC25A31 protein (arrow).)

Immunohistochemistry (IHC)

(SLC25A31 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC25A31 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (SLC25A31 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC25A31 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of SLC25A31 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of SLC25A31 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-SLC25A31 antibody
Mitochondrial ADP/ATP carriers, such as SLC25A31, are nuclear-coded mitochondrial proteins that catalyze the exchange of ATP generated in mitochondria by ATP synthase (see MIM 108729) against ADP produced in cytosol by most energy-consuming reactions (Dolce et al., 2005 [PubMed 15670820]).
Product Categories/Family for anti-SLC25A31 antibody
References
Gallerne, C., et al. Int. J. Biochem. Cell Biol. 42(5):623-629(2010). Kim, Y.H., et al. Dev. Biol. 302(2):463-476(2007). Dolce, V., et al. FEBS Lett. 579(3):633-637(2005). Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,022 Da
NCBI Official Full Name
ADP/ATP translocase 4
NCBI Official Synonym Full Names
solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 31
NCBI Official Symbol
SLC25A31
NCBI Official Synonym Symbols
AAC4; ANT4; SFEC35kDa
NCBI Protein Information
ADP/ATP translocase 4; ANT 4; ADP,ATP carrier protein 4; adenine nucleotide translocase 4; adenine nucleotide translocator 4; solute carrier family 25 member 31; sperm flagellar energy carrier protein
UniProt Protein Name
ADP/ATP translocase 4
Protein Family
UniProt Gene Name
SLC25A31
UniProt Synonym Gene Names
AAC4; ANT4; SFEC; ANT 4
UniProt Entry Name
ADT4_HUMAN

NCBI Description

Mitochondrial ADP/ATP carriers, such as SLC25A31, are nuclear-coded mitochondrial proteins that catalyze the exchange of ATP generated in mitochondria by ATP synthase (see MIM 108729) against ADP produced in cytosol by most energy-consuming reactions (Dolce et al., 2005 [PubMed 15670820]).[supplied by OMIM, Mar 2008]

Uniprot Description

SLC25A31: Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. May serve to mediate energy generating and energy consuming processes in the distal flagellum, possibly as a nucleotide shuttle between flagellar glycolysis, protein phosphorylation and mechanisms of motility. Belongs to the mitochondrial carrier family.

Protein type: Mitochondrial; Transporter; Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4q28.1

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane; nucleus

Molecular Function: transporter activity

Biological Process: transmembrane transport

Research Articles on SLC25A31

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Product Notes

The SLC25A31 slc25a31 (Catalog #AAA6348255) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLC25A31, ID (SLC25A31, AAC4, ANT4, SFEC, ADP/ATP translocase 4, ADP,ATP carrier protein 4, Adenine nucleotide translocator 4, Solute carrier family 25 member 31, Sperm flagellar energy carrier protein) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SLC25A31 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:10-50 ELISA: 1:1,000 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SLC25A31 slc25a31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC25A31, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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