Rabbit anti-Human FGF1 Polyclonal Antibody | anti-FGF1 antibody

FGF1, NT (Heparin-binding Growth Factor 1, HBGF-1, Acidic Fibroblast Growth Factor, aFGF, Beta-endothelial Cell Growth Factor, ECGF-beta, FGFA) (HRP)

Cat. Num. AAA6476215

• Gene Names: FGF1; AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: FGF1; Polyclonal Antibody; NT (Heparin-binding Growth Factor 1; HBGF-1; Acidic Fibroblast Growth Factor; aFGF; Beta-endothelial Cell Growth Factor; ECGF-beta; FGFA) (HRP); anti-FGF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FGF1.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FGF1 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 5-30 in the N-terminal region of human FGF1, UniProt Accession #P05230. Species sequence homology: porcine
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of FGF1 (arrow) in 293 cell lysates (2 ug/lane) using MBS641357 Lane 1: nontransfected Lane 2: transiently transfected with the FGF1 gene)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human colon carcinoma tissue usingMBS641357, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS641357 for Immunohistochemistry.)

Testing Data

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Product Categories/Family for anti-FGF1 antibody

Antibodies; Growth Factors; Cytokines

References

1. Fukushima, S., Int. J. Oncol. 32(2), 467-473 (2008). 2. Riley, B.M.Am. J. Med. Genet. A 143(24), 3228-3234 (2007). 3. Tomaszewski, M., Circulation 116(17), 1915-1924 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4503697
• NCBI GeneID: 2246
• NCBI Accession #: NP_000791
• NCBI GenBank Nucleotide #: NM_000800.4
• UniProt Accession #: P05230; P07502; Q16588; B2R5T0; D3DQF2
• Molecular Weight: 15.9kDa (141aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: fibroblast growth factor 1 isoform 1
• NCBI Official Synonym Full Names: fibroblast growth factor 1 (acidic)
• NCBI Official Symbol: FGF1
• NCBI Official Synonym Symbols: AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
• NCBI Protein Information: fibroblast growth factor 1; beta-endothelial cell growth factor; endothelial cell growth factor, alpha; endothelial cell growth factor, beta; heparin-binding growth factor 1
• UniProt Protein Name: Fibroblast growth factor 1
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF1
• UniProt Synonym Gene Names: FGFA; FGF-1; aFGF; ECGF; HBGF-1
• UniProt Entry Name: FGF1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]

Uniprot Description

FGF1: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell development/differentiation; Cytokine

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; extracellular space; proteinaceous extracellular matrix; extracellular region; nucleolus; cell cortex; cytosol

Molecular Function: heparin binding; protein binding; growth factor activity; Hsp70 protein binding; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; anatomical structure morphogenesis; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; multicellular organismal development; positive regulation of cholesterol biosynthetic process; signal transduction; positive regulation of MAP kinase activity; positive regulation of angiogenesis; induction of an organ; positive regulation of cell division; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of epithelial cell proliferation; lung development; positive regulation of cell migration