Western Blot (WB)
(All lanes : Anti-USP47 Antibody (Center) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: human testis lysatesLane 3: U-87 MG whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 157 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human USP47 Polyclonal Antibody | anti-USP47 antibody
USP47 Antibody (Center)
Western Blot (WB)
(All lanes : Anti-USP47 Antibody (Center) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: human testis lysatesLane 3: U-87 MG whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 157 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-USP47 Antibody (Center) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: human testis lysatesLane 3: U-87 MG whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 157 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
NCBI Description
USP47: Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulates CDC25A expression at a transcriptional level. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; EC 3.4.19.12; Actin-binding; Ubiquitin conjugating system; Motility/polarity/chemotaxis; Ubiquitin-specific protease
Chromosomal Location of Human Ortholog: 11p15.3
Cellular Component: cytoplasm; SCF ubiquitin ligase complex; nucleus
Molecular Function: protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity
Biological Process: response to drug; proteasomal ubiquitin-dependent protein catabolic process; base-excision repair; negative regulation of caspase activity; negative regulation of transcription, DNA-dependent; positive regulation of cell growth; response to DNA damage stimulus; negative regulation of apoptosis
Uniprot Description
USP47: Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulates CDC25A expression at a transcriptional level. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system; EC 3.4.19.12; Motility/polarity/chemotaxis; Actin-binding; Protease; Ubiquitin-specific protease
Chromosomal Location of Human Ortholog: 11p15.3
Cellular Component: cytoplasm; nucleus; SCF ubiquitin ligase complex
Molecular Function: protein binding; ubiquitin-specific protease activity
Biological Process: base-excision repair; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of transcription, DNA-dependent; positive regulation of cell growth; response to DNA damage stimulus; response to drug; ubiquitin-dependent protein catabolic process
Research Articles on USP47
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Product Notes
The USP47 usp47 (Catalog #AAA9215904) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The USP47 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP47 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:2000. Researchers should empirically determine the suitability of the USP47 usp47 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP47, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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