Western Blot (WB)
(Figure 1. Western blot analysis of SLC22A3 using anti-SLC22A3 antibody (MBS1753676).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: mouse skeletal muscle tissue lysatesLane 4: mouse lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SLC22A3 antigen affinity purified polyclonal antibody (Catalog # MBS1753676) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SLC22A3 at approximately 61KD. The expected band size for SLC22A3 is at 61KD. )
Rabbit anti-Human, Mouse SLC22A3 Polyclonal Antibody | anti-SLC22A3 antibody
Anti-SLC22A3 Antibody
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of SLC22A3 using anti-SLC22A3 antibody (MBS1753676).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: mouse skeletal muscle tissue lysatesLane 4: mouse lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SLC22A3 antigen affinity purified polyclonal antibody (Catalog # MBS1753676) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SLC22A3 at approximately 61KD. The expected band size for SLC22A3 is at 61KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of SLC22A3 using anti-SLC22A3 antibody (MBS1753676).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human Hela whole cell lysatesLane 3: mouse skeletal muscle tissue lysatesLane 4: mouse lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SLC22A3 antigen affinity purified polyclonal antibody (Catalog # MBS1753676) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for SLC22A3 at approximately 61KD. The expected band size for SLC22A3 is at 61KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of Hela cells using anti-SLC22A3 antibody (MBS1753676).Overlay histogram showing Hela cells stained with MBS1753676 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC22A3 Antibody (MBS1753676, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of Hela cells using anti-SLC22A3 antibody (MBS1753676).Overlay histogram showing Hela cells stained with MBS1753676 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC22A3 Antibody (MBS1753676, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Nagano, T., Mitchell, J. A., Sanz, L. A., Pauler, F. M., Ferguson-Smith, A. C., Feil, R., Fraser, P. The Air noncoding RNA epigenetically silences transcription by targeting G9a to chromatin. Science 322: 1717-1720, 2008.
3. Sleutels, F., Zwart, R., Barlow, D. P. The non-coding Air RNA is required for silencing autosomal imprinted genes. Nature 415: 810-813, 2002.
NCBI and Uniprot Product Information
NCBI Description
Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. [provided by RefSeq, Jul 2008]
Uniprot Description
SLC22A3: Mediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain. Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.
Protein type: Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral
Chromosomal Location of Human Ortholog: 6q25.3
Cellular Component: membrane; integral to plasma membrane; plasma membrane
Molecular Function: protein binding; toxin transporter activity; organic cation transmembrane transporter activity; dopamine transmembrane transporter activity; quaternary ammonium group transmembrane transporter activity
Biological Process: dopamine transport; organic cation transport; histamine uptake; quaternary ammonium group transport; regulation of appetite; multidrug transport; transmembrane transport
Research Articles on SLC22A3
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Product Notes
The SLC22A3 slc22a3 (Catalog #AAA1753676) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SLC22A3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SLC22A3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS/FCM). WB: 0.25-0.5ug/ml|Human, Mouse| FC/FACS/FCM: 1-3ug/1x106 cells|Human|. Researchers should empirically determine the suitability of the SLC22A3 slc22a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC22A3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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