Rabbit SKAP1 Polyclonal Antibody | anti-SKAP1 antibody

SKAP1 Antibody

Cat. Num. AAA9629785

• Gene Names: SKAP1; SCAP1; SKAP55; HEL-S-81p
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SKAP1; Polyclonal Antibody; SKAP1 Antibody; pp55; SCAP 1; SCAP1; SKAP 1; SKAP 55; SKAP-55; Skap1; SKAP1_HUMAN; SKAP55 adaptor protein; SRC family associated phosphoprotein 1; Src family-associated phosphoprotein 1; Src kinase associated phosphoprotein 1; SRC kinase associated phosphoprotein 55 kD; Src kinase associated phosphoprotein of 55 kDa; Src kinase-associated phosphoprotein 1; Src kinase-associated phosphoprotein of 55 kDa; anti-SKAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SKAP1 Antibody detects endogenous levels of SKAP1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SKAP1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human SKAP1.
• Conjugation: Unconjugated
• Similarity: The SH3 domain interacts with FYB1. Belongs to the SKAP family.
• Post Translational Modifications: Phosphorylated on tyrosines. Phosphorylation by FYN on Tyr-271 is required for GRB2 interaction. Phosphorylation by FYN on Tyr-295 abolishes interaction with FYB1. Tyr-232 is dephosphorylated by PTPRC (Probable).
• Subunit Structure: Homodimer. Interacts with FYN. Interacts with PTPRC. Interacts with GRB2 when phosphorylated on Tyr-271. Interacts with FYB1, which is required for SKAP2 protein stability. Part of a complex consisting of SKAP1, FYB1 and CLNK (By similarity). Interacts with RASGRP1. Interacts with FYB2.
• Tissue Specificity: Highly expressed in thymocytes and peripheral blood lymphocytes. Also expressed in spleen cells and testis. Present in T-cells (at protein level).
• Function: Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.
• Note: Upon T-cell stimulation, translocates to lipid rafts at the cell membrane.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-SKAP1 antibody

Cytoplasm; Nucleus; Cell membrane; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 269849660
• NCBI GeneID: 8631
• UniProt Accession #: Q86WV1; O15268; D3DTV1
• Molecular Weight: 359
• NCBI Official Full Name: Src kinase-associated phosphoprotein 1
• NCBI Official Synonym Full Names: src kinase associated phosphoprotein 1
• NCBI Official Symbol: SKAP1
• NCBI Official Synonym Symbols: SCAP1; SKAP55; HEL-S-81p
• NCBI Protein Information: src kinase-associated phosphoprotein 1; pp55; SKAP-55; src family associated phosphoprotein 1; src family-associated phosphoprotein 1; src kinase-associated phosphoprotein of 55 kDa; epididymis secretory sperm binding protein Li 81p
• UniProt Protein Name: Src kinase-associated phosphoprotein 1
• Protein Family: Src kinase-associated phosphoprotein
• UniProt Gene Name: SKAP1
• UniProt Synonym Gene Names: SCAP1; SKAP55; SKAP-55; pp55
• UniProt Entry Name: SKAP1_HUMAN

NCBI Description

This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins. [provided by RefSeq, Jul 2008]

Uniprot Description

SKAP55: Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells. Belongs to the SKAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: cytoplasm; plasma membrane; intercellular junction; nucleus

Molecular Function: protein binding; SH3/SH2 adaptor activity; protein complex binding; SH2 domain binding; SH3 domain binding; protein phosphatase binding; protein kinase binding

Biological Process: positive regulation of signal transduction; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; T cell receptor signaling pathway