Rabbit anti-Human SKA1 Polyclonal Antibody | anti-SKA1 antibody

SKA1, CT (Spindle and Kinetochore-associated Protein 1, C18orf24)

Cat. Num. AAA641659

• Gene Names: SKA1; C18orf24
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: SKA1; Polyclonal Antibody; CT (Spindle and Kinetochore-associated Protein 1; C18orf24); Anti -SKA1; anti-SKA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human SKA1. Species sequence homology: Mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-SKA1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.5-1ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to human SKA1 at C-terminal conjugated to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cerebellum: Formalin-Fixed, Paraffin- Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Brain, Cortex: Formalin- Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of SKA1 in A549 cell lysate with SKA1 antibody at (A) 0.5 and (B) 1ug/ml.)

Related Product Information for anti-SKA1 antibody

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules.

Product Categories/Family for anti-SKA1 antibody

Antibodies; Abs to DNA/RNA Translation

NCBI and Uniprot Product Information

• NCBI GI #: 88014625
• NCBI GeneID: 220134
• NCBI Accession #: NP_001034624.1
• NCBI GenBank Nucleotide #: NM_001039535.2
• UniProt Accession #: Q96BD8; B2R9Y6; B4E0P4
• Molecular Weight: 29,484 Da
• NCBI Official Full Name: spindle and kinetochore-associated protein 1
• NCBI Official Synonym Full Names: spindle and kinetochore associated complex subunit 1
• NCBI Official Symbol: SKA1
• NCBI Official Synonym Symbols: C18orf24
• NCBI Protein Information: spindle and kinetochore-associated protein 1; spindle and KT (kinetochore) associated 1
• UniProt Protein Name: Spindle and kinetochore-associated protein 1
• Protein Family: Spindle and kinetochore-associated protein
• UniProt Gene Name: SKA1
• UniProt Synonym Gene Names: C18orf24
• UniProt Entry Name: SKA1_HUMAN

Uniprot Description

SKA1: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules. Belongs to the SKA1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: microtubule cytoskeleton; spindle microtubule; outer kinetochore of condensed chromosome; cytosol

Molecular Function: protein binding; microtubule binding

Biological Process: mitosis; cell division; mitotic cell cycle; regulation of microtubule polymerization or depolymerization; chromosome segregation