Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Sirtuin 5 (SIRT5) Polyclonal Antibody | anti-SIRT5 antibody
Polyclonal Antibody to Sirtuin 5 (SIRT5)
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Sirtuin 5 (SIRT5); Polyclonal Antibody; Polyclonal Antibody to Sirtuin 5 (SIRT5); anti-SIRT5 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against SIRT5. It has been selected for its ability to recognize SIRT5 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- ANA KHIVIISGAG VSAESGVPTF RGTGGYWRKW QAQHLATPLA FAHNPSQVWE FYHYRREVMR NKEPNPGHLA IAQCEARLRD QGRRVVVITQ NIDELHRKAG TKNLLEIHGT LFKTRCTSCG NVAENYKSPI CPALLGKGAP EPDTQESRIP VHKLPRCEEA GCGGLLRPHV VWFGENLDPA ILKEVDRELA RCDL
MGHHHHHHSGSEF- ANA KHIVIISGAG VSAESGVPTF RGTGGYWRKW QAQHLATPLA FAHNPSQVWE FYHYRREVMR NKEPNPGHLA IAQCEARLRD QGRRVVVITQ NIDELHRKAG TKNLLEIHGT LFKTRCTSCG NVAENYKSPI CPALLGKGAP EPDTQESRIP VHKLPRCEEA GCGGLLRPHV VWFGENLDPA ILKEVDRELA RCDL
Applicable Applications for anti-SIRT5 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant SIRT5 (Ala48~Leu244) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2075827
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,098 Da
NCBI Official Full Name
NAD-dependent protein deacylase sirtuin-5, mitochondrial
NCBI Official Synonym Full Names
sirtuin 5
NCBI Official Symbol
Sirt5
NCBI Protein Information
NAD-dependent protein deacylase sirtuin-5, mitochondrial
UniProt Protein Name
NAD-dependent protein deacylase sirtuin-5, mitochondrial
Protein Family
UniProt Gene Name
Sirt5
Uniprot Description
SIRT5: NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Has weak NAD- dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro. Belongs to the sirtuin family. Class III subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Deacetylase; EC 3.5.1.-; Mitochondrial
Chromosomal Location of Human Ortholog: 17p12
Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial matrix; mitochondrion; nucleus
Molecular Function: zinc ion binding
Biological Process: protein deacetylation; response to nutrient levels
Research Articles on SIRT5
Similar Products
Product Notes
The SIRT5 sirt5 (Catalog #AAA2006474) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Sirtuin 5 (SIRT5) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Sirtuin 5 (SIRT5) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SIRT5 sirt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- ANA KHIVIISGAG VSAESGVPTF RGTGGYWRKW QAQHLATPLA FAHNPSQVWE FYHYRREVMR NKEPNPGHLA IAQCEARLRD QGRRVVVITQ NIDELHRKAG TKNLLEIHGT LFKTRCTSCG NVAENYKSPI CPALLGKGAP EPDTQESRIP VHKLPRCEEA GCGGLLRPHV VWFGENLDPA ILKEVDRELA RCDL. It is sometimes possible for the material contained within the vial of "Sirtuin 5 (SIRT5), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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