Rabbit anti-Human SIRT6 / Sirtuin 6 Polyclonal Antibody | anti-SIRT6 antibody

Rabbit Polyclonal to Human SIRT6 / Sirtuin 6

Cat. Num. AAA241080

• Gene Names: SIRT6; SIR2L6
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Protein G Purified
• Synonyms: SIRT6 / Sirtuin 6; Polyclonal Antibody; Rabbit Polyclonal to Human SIRT6 / Sirtuin 6; Anti-SIRT6 / Sirtuin 6 Antibody (aa305-320) IHC-plus; SIRT6; SIR2-like protein 6; Sirtuin type 6; SIR2L6; Sir2-related protein type 6; Sirtuin 6; Human SIRT6; anti-SIRT6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Amino acids 305 to 320 of human SIRT6
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.09% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 355

• Applicable Applications for anti-SIRT6 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:000 - 1:1000), IHC-P (10 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human SIRT6.
• Immunogen Type: Synthetic peptide
• Immunogen: SIRT6 / Sirtuin 6 antibody was raised against synthetic peptide from human SIRT6.
• Antigen Modification: aa305-320
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-SIRT6 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 7706710
• NCBI GeneID: 51548
• NCBI Accession #: NP_057623.1
• NCBI GenBank Nucleotide #: NM_016539.1
• UniProt Accession #: Q8N6T7; O75291; Q6IAF5; Q6PK99; Q8NCD2; Q9BSI5; Q9BWP3; Q9NRC7; Q9UQD1; B2RCD0
• Molecular Weight: 36,065 Da
• NCBI Official Full Name: NAD-dependent deacetylase sirtuin-6
• NCBI Official Synonym Full Names: sirtuin 6
• NCBI Official Symbol: SIRT6
• NCBI Official Synonym Symbols: SIR2L6
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-6; sirtuin type 6; SIR2-like protein 6; sir2-related protein type 6; regulatory protein SIR2 homolog 6; NAD-dependent deacetylase sirtuin-6
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-6
• Protein Family: NAD-dependent protein deacetylase
• UniProt Gene Name: SIRT6
• UniProt Synonym Gene Names: SIR2L6
• UniProt Entry Name: SIR6_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jul 2010]

Uniprot Description

SIRT6: NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. Interacts with RELA. Interacts with RBBP8; the interaction deacetylates RBBP8. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.-; EC 2.4.2.31; Transferase; Deacetylase

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleolus; nuclear telomeric heterochromatin; nucleus

Molecular Function: NAD(P)+-protein-arginine ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; zinc ion binding; NAD-dependent histone deacetylase activity; chromatin binding; NAD+ ADP-ribosyltransferase activity

Biological Process: protein amino acid ADP-ribosylation; histone deacetylation