Rabbit anti-Human HIPK2 Polyclonal Antibody | anti-HIPK2 antibody

HIPK2, Phosphorylated (Tyr361) (Homeodomain Interacting Protein Kinase 2, hHIPk2, DKFZp686K02111, FLJ23711, PRO0593) (Biotin)

Cat. Num. AAA6480406

• Gene Names: HIPK2; PRO0593
• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HIPK2; Polyclonal Antibody; Phosphorylated (Tyr361) (Homeodomain Interacting Protein Kinase 2; hHIPk2; DKFZp686K02111; FLJ23711; PRO0593) (Biotin); EC=2.7.11.1; Nuclear Body-associated Kinase 1; Nbak1; Sialophorin Tail-associated Nuclear Serine/threonine-protein Kinase; Stank; anti-HIPK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HIPK2 when phosphorylated at Tyr361.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-HIPK2 antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr361 of human HIPK2 (KLH).
• Conjugate: Biotin
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-HIPK2-pY361 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-HIPK2 antibody

Antibodies; Protein Kinases

References

1. Guha U, et al. (2008). Proc Natl Acad Sci USA 105, 14112-7. 2. Daub H, et al. (2008). Mol Cell 31, 438-48. 3. Guo A, et al. (2008). Proc Natl Acad Sci USA 105, 692-7. 4. Rikova K, et al. (2007). Cell 131, 1190-203.

NCBI and Uniprot Product Information

• NCBI GI #: 164420685
• NCBI GeneID: 28996
• NCBI Accession #: NP_073577
• NCBI GenBank Nucleotide #: NM_022740
• UniProt Accession #: Q9H2X6
• Molecular Weight: 120kDa
• NCBI Official Full Name: homeodomain-interacting protein kinase 2 isoform 1
• NCBI Official Synonym Full Names: homeodomain interacting protein kinase 2
• NCBI Official Symbol: HIPK2
• NCBI Official Synonym Symbols: PRO0593
• NCBI Protein Information: homeodomain-interacting protein kinase 2
• UniProt Protein Name: Homeodomain-interacting protein kinase 2
• Protein Family: Homeodomain-interacting protein kinase
• UniProt Gene Name: HIPK2
• UniProt Synonym Gene Names: hHIPk2
• UniProt Entry Name: HIPK2_HUMAN

NCBI Description

This gene encodes a conserved serine/threonine kinase that is a member of the homeodomain-interacting protein kinase family. The encoded protein interacts with homeodomain transcription factors and many other transcription factors such as p53, and can function as both a corepressor and a coactivator depending on the transcription factor and its subcellular localization. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

HIPK2: homeodomain interacting protein kinase 2 is a CMGC kinase of the DYRK family. Interacts with TRADD. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Inhibits cell growth and promotes apoptosis. Involved in transcriptional activation of TP53 and TP73. In response to TGFB, cooperates with DAXX to activate JNK. Phosphorylates the antiapoptotic factor CTBP1 and promotes its proteasomal degradation. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between TAK1 and NLK to promote the proteasomal degradation of MYB. Highly expressed in neuronal tissues, heart and kidney. Weakly expressed ubiquitously. Possible tumor suppressor. Activates p53 in response to UV light, causing growth arrest and apoptosis. Required for cisplatin-induced apoptosis. Downregulated in breast and thyroid carcinomas. Three alternatively spliced isoforms have been described.

Protein type: Protein kinase, CMGC; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; DYRK family; HIPK subfamily

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: nuclear body; PML body; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; virion binding; SMAD binding; transcription corepressor activity; ATP binding; protein kinase activity

Biological Process: voluntary musculoskeletal movement; positive regulation of protein binding; regulation of cell cycle; positive regulation of transcription, DNA-dependent; positive regulation of JNK cascade; PML body organization and biogenesis; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; adult walking behavior; negative regulation of BMP signaling pathway; neuron differentiation; anterior/posterior pattern formation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; erythrocyte differentiation; negative regulation of neuron apoptosis; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; positive regulation of DNA binding; smoothened signaling pathway; transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; peptidyl-threonine phosphorylation; peptidyl-serine phosphorylation; positive regulation of angiogenesis; eye development; virus-host interaction; embryonic retina morphogenesis in camera-type eye; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; embryonic camera-type eye morphogenesis; positive regulation of protein amino acid phosphorylation