Western Blot (WB)
(Western blot analysis of SIN3A expression in Hela (A), MCF7 (B), COS7 (C) whole cell lysates.)
Rabbit SIN3A Polyclonal Antibody | anti-SIN3A antibody
Anti-SIN3A Antibody
Western Blot (WB)
(Western blot analysis of SIN3A expression in Hela (A), MCF7 (B), COS7 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of SIN3A expression in Hela (A), MCF7 (B), COS7 (C) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of SIN3A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of SIN3A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex. [provided by RefSeq, Jul 2008]
Uniprot Description
SIN3A: Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Interacts with BAZ2A, MXD3, MXD4, MBD2, NCOR1, NR4A2, REST, RLIM, SAP30, SETDB1 and SMYD2. Interacts with PHF12 in a complex composed of HDAC1, PHF12 and SAP30. Interacts with ARID4B, BRMS1L, DACH1, HCFC1, HDAC1, HDAC2, MXI1, SAP30L, SAP130, SFPQ, SUDS3 and TOPORS. Interacts with TET1; the interaction recruits SIN3A to gene promoters. Interacts with OGT (via TPRs 1-6); the interaction mediates transcriptional repression in parallel with histone deacetylase.
Protein type: Nuclear receptor co-regulator; Nucleolus; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 15q24.2
Cellular Component: cytoplasm; intercellular bridge; kinetochore; nucleolus; nucleoplasm; nucleus; Sin3 complex; transcription factor complex; transcriptional repressor complex
Molecular Function: chromatin binding; histone deacetylase activity; protein binding; protein complex binding; protein deacetylase activity; RNA binding; transcription factor activity
Biological Process: activation of innate immune response; aging; cellular lipid metabolic process; DNA replication; hemopoietic progenitor cell differentiation; histone deacetylation; in utero embryonic development; negative regulation of apoptosis; negative regulation of circadian rhythm; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of chromatin silencing; positive regulation of defense response to virus by host; positive regulation of transcription from RNA polymerase II promoter; protein amino acid deacetylation; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; response to methylglyoxal; rhythmic process; transcription, DNA-dependent
Research Articles on SIN3A
Similar Products
Product Notes
The SIN3A sin3a (Catalog #AAA8245189) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SIN3A Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SIN3A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP). WB: 1/500 - 1/2000; IF/ICC: 1/50 - 1/200; IP: 1/50 - 1/200; ChIP: 1/20 - 1/100. Researchers should empirically determine the suitability of the SIN3A sin3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIN3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
