Mouse Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2) ELISA Kit | MYH2 elisa kit

Mouse Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2) ELISA Kit

Cat. Num. AAA2022522

• Gene Names: MYH2; IBM3; MYH2A; MYPOP; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa
• Reactivity: Mouse
• Synonyms: Myosin Heavy Chain 2; Skeletal Muscle; Adult (MYH2); Mouse Myosin Heavy Chain 2; Adult (MYH2) ELISA Kit; MYH2A; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa; MYH2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2).; No significant cross-reactivity or interference between Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2) and analogues was observed.
• Sequence Length: 1941

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.121ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYH2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MYH2 elisa kit

Metabolic pathway; Developmental science; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 153791586
• NCBI GeneID: 4620
• NCBI Accession #: NP_001093582.1
• NCBI GenBank Nucleotide #: NM_001100112.1
• Molecular Weight: 79,880 Da
• NCBI Official Full Name: myosin-2
• NCBI Official Synonym Full Names: myosin, heavy chain 2, skeletal muscle, adult
• NCBI Official Symbol: MYH2
• NCBI Official Synonym Symbols: IBM3; MYH2A; MYPOP; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa
• NCBI Protein Information: myosin-2
• UniProt Protein Name: Myosin-2
• UniProt Gene Name: MYH2
• UniProt Synonym Gene Names: MYHSA2; MyHC-2a; MyHC-IIa
• UniProt Entry Name: MYH2_HUMAN

NCBI Description

Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009]

Uniprot Description

MYH2: Muscle contraction. Required for cytoskeleton organization. Defects in MYH2 are the cause of inclusion body myopathy type 3 (IBM3). Hereditary inclusion body myopathies constitute a group of neuromuscular disorders characterized by slowly progressive distal and proximal weakness and a typical muscle pathology including rimmed vacuoles and filamentous inclusions. IBM3 is a variant of hereditary inclusion body myopathies and is characterized by autosomal dominant myopathy with joint contracture, ophthalmoplegia and rimmed vacuoles. Morphological analysis of muscle biopsies from patients indicate that the type 2A fibers frequently were abnormal, whereas other fiber types appeared normal.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: Golgi apparatus; sarcomere; protein complex; myofibril; contractile ring; muscle myosin complex; intercellular junction; cytosol; A band

Molecular Function: calmodulin binding; microfilament motor activity; protein binding; actin binding; ATP binding

Biological Process: muscle contraction; metabolic process; plasma membrane repair; innate immune response; response to activity; muscle filament sliding

Disease: Inclusion Body Myopathy 3, Autosomal Dominant