Western Blot (WB)
(Western blot analysis of extracts from MCF7, using Phospho-SIN3A(Ser832) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Rabbit SIN3A Polyclonal Antibody | anti-SIN3A antibody
Phospho-SIN3A (Ser832) Antibody
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (83%), Xenopus (86%)
Applications
Western Blot, ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
SIN3A; Polyclonal Antibody; Phospho-SIN3A (Ser832) Antibody; AW553200; DKFZP434K2235; FLJ90319; Histone deacetylase complex subunit Sin 3a; Histone deacetylase complex subunit Sin3a; KIAA0700; Kiaa4126; mKIAA4126; Paired amphipathic helix protein Sin 3a; Paired amphipathic helix protein Sin3a; Sin 3a; SIN3 homolog A; SIN3 homolog A transcription regulator (yeast); SIN3 homolog A transcription regulator; SIN3 transcription regulator homolog A; Sin3a; SIN3A protein; SIN3A_HUMAN; Transcriptional co repressor Sin 3A; Transcriptional co repressor Sin3A; Transcriptional corepressor Sin3a; Transcriptional regulator SIN3A; anti-SIN3A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (83%), Xenopus (86%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (83%), Xenopus (86%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-SIN3A (Ser832) Antibody detects endogenous levels of SIN3A only when phosphorylated at Ser832.
Tissue Specificity: Expressed in the developing brain, with highest levels of expression detected in the ventricular zone of various cortical regions.
Tissue Specificity: Expressed in the developing brain, with highest levels of expression detected in the ventricular zone of various cortical regions.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-SIN3A antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human SIN3A around the phosphorylation site of Ser832.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
SUMO1 sumoylated by TOPORS. Probably desumoylated by SENP2.
Subunit Structure
Interacts with ARID4B, BRMS1L, HCFC1, HDAC1, HDAC2, MXI1, SAP30L, SAP130, SFPQ and TOPORS. Interacts with OGT (via TPRs 1-6); the interaction mediates transcriptional repression in parallel with histone deacetylase. Interacts with BAZ2A, MXD1, MXD3, MXD4, MBD2, DACH1, NCOR1, NR4A2, REST, RLIM, SAP30, SETDB1, SMYD2, and SUDS3. Interacts with PHF12 in a complex composed of HDAC1, PHF12 and SAP30 (By similarity). Interacts with TET1; the interaction recruits SIN3A to gene promoters. The large PER complex involved in the histone deacetylation is composed of at least HDAC1, PER2, SFPQ and SIN3A (By similarity). Interacts with KLF11 (By similarity). Interacts with PPHLN1. Found in a complex with YY1, GON4L and HDAC1 (By similarity). Interacts (via PAH2) with FOXK1 (By similarity). Interacts with FOXK2 (By similarity). Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1. Interacts with SINHCAF (By similarity). Interacts with SPHK2.
Subcellular Location
Nucleus. Nucleus>Nucleolus.
Note: Recruited to the nucleolus by SAP30L.
Note: Recruited to the nucleolus by SAP30L.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from MCF7, using Phospho-SIN3A(Ser832) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from MCF7, using Phospho-SIN3A(Ser832) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Related Product Information for anti-SIN3A antibody
Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression (By similarity). Required for cortical neuron differentiation and callosal axon elongation (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
1273
NCBI Official Full Name
paired amphipathic helix protein Sin3a
NCBI Official Synonym Full Names
SIN3 transcription regulator family member A
NCBI Official Symbol
SIN3A
NCBI Protein Information
paired amphipathic helix protein Sin3a; transcriptional regulator, SIN3A; transcriptional corepressor Sin3a; transcriptional co-repressor Sin3A; SIN3 transcription regulator homolog A; SIN3 homolog A, transcription regulator; histone deacetylase complex s
UniProt Protein Name
Paired amphipathic helix protein Sin3a
Protein Family
UniProt Gene Name
SIN3A
UniProt Entry Name
SIN3A_HUMAN
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Product Notes
The SIN3A sin3a (Catalog #AAA9613113) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-SIN3A (Ser832) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (83%), Xenopus (86%) and may cross-react with other species as described in the data sheet. AAA Biotech's SIN3A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the SIN3A sin3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIN3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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