Western Blot (WB)
((1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SIM1 Polyclonal Antibody | anti-SIM1 antibody
Goat anti-SIM1 Antibody
Western Blot: Approx 80kDa band observed in lysates of cell line HEK293 (calculated MW of 85.5kDa according to NP_005059.2). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. SIM1 transcript was detected only in fetal kidney out of various adult and fetal tissues tested. Since the sim gene plays an important role in Drosophila development and has peak levels of expression during the period of neurogenesis,it was proposed that the human SIM gene is a candidate for involvement in certain dysmorphic features (particularly the facial and skull characteristics), abnormalities of brain development, and/or mental retardation of Down syndrome. [provided by RefSeq, Jul 2008]
Uniprot Description
SIM1: Transcriptional factor that may have pleiotropic effects during embryogenesis and in the adult.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 6q16.3
Cellular Component: nucleus
Molecular Function: DNA binding; protein heterodimerization activity; transcription factor activity
Biological Process: cell differentiation; nervous system development; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; ureteric bud development
Disease: Obesity
Research Articles on SIM1
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Product Notes
The SIM1 sim1 (Catalog #AAA422883) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SIM1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's SIM1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 2000. Western Blot: Approx 80kDa band observed in lysates of cell line HEK293 (calculated MW of 85.5kDa according to NP_005059.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the SIM1 sim1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SGDRYRTEQY QS. It is sometimes possible for the material contained within the vial of "SIM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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