Western Blot (WB)
(Western blot analysis of anti-SIGLEC7 Pab in NCI-H460 cell line lysate (35ug/lane). SIGLEC7 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse Siglec 7 Polyclonal Antibody | anti-SIGLEC7 antibody
Siglec 7, NT (Sialic Acid-binding Ig-like Lectin 7, Siglec 7, QA79 Membrane Protein, Adhesion Inhibitory Receptor Molecule-1, AIRM-1, p75, D-siglec, AIRM1, D-siglec, CDw328, CD328) (AP)
Gene Names
SIGLEC7; p75; QA79; AIRM1; CD328; CDw328; D-siglec; SIGLEC-7; SIGLECP2; SIGLEC19P; p75/AIRM1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
Siglec 7; Polyclonal Antibody; NT (Sialic Acid-binding Ig-like Lectin 7; QA79 Membrane Protein; Adhesion Inhibitory Receptor Molecule-1; AIRM-1; p75; D-siglec; AIRM1; CDw328; CD328) (AP); anti-SIGLEC7 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SIGLEC7. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Sequence Length
467
Applicable Applications for anti-SIGLEC7 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human D-SIGLEC (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of anti-SIGLEC7 Pab in NCI-H460 cell line lysate (35ug/lane). SIGLEC7 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-SIGLEC7 Pab in NCI-H460 cell line lysate (35ug/lane). SIGLEC7 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Product Categories/Family for anti-SIGLEC7 antibody
References
1. Nicoll, G., et al., Eur. J. Immunol. 33(6):1642-1648 (2003). 2. Alphey, M.S., et al., J. Biol. Chem. 278(5):3372-3377 (2003). 3. Angata, T., et al., Glycobiology 10(4):431-438 (2000). 4. Falco, M., et al., J. Exp. Med. 190(6):793-802 (1999). 5. Nicoll, G., et al., J. Biol. Chem. 274(48):34089-34095 (1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
51.142
NCBI Official Full Name
Sialic acid-binding Ig-like lectin 7
NCBI Official Synonym Full Names
sialic acid binding Ig like lectin 7
NCBI Official Symbol
SIGLEC7
NCBI Official Synonym Symbols
p75; QA79; AIRM1; CD328; CDw328; D-siglec; SIGLEC-7; SIGLECP2; SIGLEC19P; p75/AIRM1
NCBI Protein Information
sialic acid-binding Ig-like lectin 7
Protein Family
Research Articles on SIGLEC7
Similar Products
Product Notes
The SIGLEC7 (Catalog #AAA6499430) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Siglec 7, NT (Sialic Acid-binding Ig-like Lectin 7, Siglec 7, QA79 Membrane Protein, Adhesion Inhibitory Receptor Molecule-1, AIRM-1, p75, D-siglec, AIRM1, D-siglec, CDw328, CD328) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Siglec 7 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SIGLEC7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Siglec 7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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