Goat SIAHBP1 Polyclonal Antibody | anti-PUF60 antibody

SIAHBP1 (Siah-binding Protein 1, Siah-BP1, 60kD Poly(U)-binding-splicing Factor, FUSE-binding Protein-interacting Repressor, FBP-interacting Repressor, FIR, FLJ31379, Poly-U binding Splicing Factor 60kDa, Poly(U)-binding-splicing Factor PUF60, PUF60, Ro-b

Cat. Num. AAA6006177

• Gene Names: PUF60; FIR; RoBPI; SIAHBP1
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: SIAHBP1; Polyclonal Antibody; SIAHBP1 (Siah-binding Protein 1; Siah-BP1; 60kD Poly(U)-binding-splicing Factor; FUSE-binding Protein-interacting Repressor; FBP-interacting Repressor; FIR; FLJ31379; Poly-U binding Splicing Factor 60kDa; Poly(U)-binding-splicing Factor PUF60; PUF60; Ro-b; Anti -SIAHBP1 (Siah-binding Protein 1; anti-PUF60 antibody

• Host: Goat
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human PUF60. Species sequence homology: Bovine, canine, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-PUF60 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:32,000; Western Blot: 1-3ug/ml, observed in lysate of human epidermoid carcinoma A431 on ~60kD bands
• Immunogen: Synthetic peptide corresponding to C-YDQERFDNSDLSA, from human PUF60, at C-terminal (NP_510965.1, NP_055096.2, NP_001129505.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

((1ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-PUF60 antibody

DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.

Product Categories/Family for anti-PUF60 antibody

Antibodies; Abs to Splicing Factors

NCBI and Uniprot Product Information

• NCBI GI #: 48146403
• NCBI GeneID: 22827
• UniProt Accession #: Q9UHX1; Q969E7; Q96D94; Q96H63; Q99628; Q9NZA0; Q9UJY7; A8K8K8
• Molecular Weight: 59,875 Da
• NCBI Official Full Name: SIAHBP1
• NCBI Official Synonym Full Names: poly-U binding splicing factor 60KDa
• NCBI Official Symbol: PUF60
• NCBI Official Synonym Symbols: FIR; RoBPI; SIAHBP1
• NCBI Protein Information: poly(U)-binding-splicing factor PUF60; poly(U)-binding-splicing factor PUF60; Siah binding protein 1; FBP interacting repressor; Ro ribonucleoprotein-binding protein 1; pyrimidine tract binding splicing factor; FUSE-binding protein-interacting repressor
• UniProt Protein Name: Poly(U)-binding-splicing factor PUF60
• Protein Family: Poly(U)-binding-splicing factor
• UniProt Gene Name: PUF60
• UniProt Synonym Gene Names: FIR; ROBPI; SIAHBP1; FBP-interacting repressor; RoBP1; Siah-BP1
• UniProt Entry Name: PUF60_HUMAN

NCBI Description

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

FIR: fuse-binding protein-interacting repressor. Interacts with (and may activate) Ro RNPs. Forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. Two alternatively spliced isoforms have been described.

Protein type: Spliceosome; RNA splicing; RNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm; Golgi apparatus; mitochondrion; ribonucleoprotein complex; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function: identical protein binding; protein binding; DNA binding; nucleotide binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis; RNA splicing; mRNA processing

Disease: Verheij Syndrome