Standard Curve (Sample)
Mouse Neuron-Specific Enolase ELISA Kit | NSE elisa kit
Mouse Neuron-Specific Enolase ELISA Kit
Gene Names
ENO2; NSE
Reactivity
Mouse
Synonyms
Neuron-Specific Enolase; Mouse Neuron-Specific Enolase ELISA Kit; NSE/ENO2/gamma-Enolase/enolase 2 (gamma; neuronal)/gamma-enolase/Neural enolase/neuron specific gamma enolase/Neuron-specific enolase; NSE elisa kit
Reactivity
Mouse
Specificity
Specifically recognize NSE, no obvious cross reaction with other analogues
Sequence Length
434
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
78.125-5000pg/ml
Sensitivity
46.875pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for NSE elisa kit
Background: Neuron-specific enolase (NSE) is known to be a cell specific isoenzyme of the glycolytic enzyme enolase. NSE is a highly specific marker for neurons and peripheral neuroendocrine cells. NSE is currently the most reliable tumour marker in diagnosis, prognosis and follow-up of small cell lung cancer (SCLC), even though increased levels of NSE have been reported also in non-small cell lung cancer (NSCLC). NSE has been demonstrated to provide quantitative measures of brain damage and/or to improve the diagnosis and the outcome evaluation in ischaemic stroke, intracerebral hemorrhage, seizures, comatose patients after cardiopulmonary resuscitation for cardiac arrest and traumatic brain injury.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti NSE antibody was precoated onto the 96-well plate. The biotin conjugated anti NSE antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with NSE conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of NSE in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti NSE antibody was precoated onto the 96-well plate. The biotin conjugated anti NSE antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with NSE conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of NSE in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
47,266 Da
NCBI Official Full Name
neuron-specific enolase
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE
NCBI Protein Information
enolase 2 (gamma, neuronal)
UniProt Protein Name
Neuron-specific enolase
UniProt Gene Name
NSE
UniProt Entry Name
G9JNA8_CAPHI
Similar Products
Product Notes
The Mouse NSE nse (Catalog #AAA17349) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17349 ELISA Kit recognizes Mouse NSE. It is sometimes possible for the material contained within the vial of "Neuron-Specific Enolase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
