Western Blot (WB)
(Western blot analysis of extracts from C6 or NIH/3T3 cells, starved for 18 hours, and treated with PDGF (50ng/ml) for 20 minutes, using MBS630282 (upper) or control S1013-29A (lower).)
Rabbit anti-Human SHP 2 Polyclonal Antibody
SHP 2, phosphorylated (Tyr542) (SHPTP-2, PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp, PTPN11)
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by protein A and peptide affinity chromatography.
Synonyms
SHP 2; Polyclonal Antibody; phosphorylated (Tyr542) (SHPTP-2; PTP2C; SH-PTP2; PTP1D; SH-PTP3; Syp; PTPN11); Anti -SHP 2; anti-SHP 2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous levels of human SHP-2 only when phosphorylated at Tyr542. Species Crossreactivity: mouse, rat.
Purity/Purification
Affinity Purified
Purified by protein A and peptide affinity chromatography.
Purified by protein A and peptide affinity chromatography.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Applicable Applications for anti-SHP 2 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:100
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr542 of human SHP-2. MW: 72kD
Species Sequence Homology
Chicken, Xenopus.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from C6 or NIH/3T3 cells, starved for 18 hours, and treated with PDGF (50ng/ml) for 20 minutes, using MBS630282 (upper) or control S1013-29A (lower).)
Western Blot (WB)
(Western blot analysis of extracts from C6 or NIH/3T3 cells, starved for 18 hours, and treated with PDGF (50ng/ml) for 20 minutes, using MBS630282 (upper) or control S1013-29A (lower).)
Related Product Information for anti-SHP 2 antibody
SHP2, also known as SHPTP2, Syp, PTP1D and PTP2C, is a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death (1). Activation of SHP 2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2). In addition to its role in Gab1-mediated Erk activation, SHP 2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites (3). SHP 2 becomes phosphorylated at Tyr542 and Tyr580 in its C-terminus in response to growth factor receptor activation (4). These phosphorylations are proposed to relieve basal inhibition and stimulate its tyrosine phosphatase activity (5).
Product Categories/Family for anti-SHP 2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
SHP-2
Similar Products
Product Notes
The SHP 2 (Catalog #AAA630282) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHP 2, phosphorylated (Tyr542) (SHPTP-2, PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp, PTPN11) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SHP 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100. Researchers should empirically determine the suitability of the SHP 2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHP 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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