Sheep anti Guinea Pig IgM (Fc specific) Secondary Antibody
Sheep anti Guinea Pig IgM (Fc specific)
Reactivity
Guinea Pig
Applications
Immunoelectrophoresis (IEP)
Synonyms
Sheep anti Guinea Pig IgM (Fc specific); Polyclonal Secondary Antibody; Sheep anti Guinea Pig IgM (Fc specific) secondary antibody
Host
Sheep
Reactivity
Guinea Pig
Clonality
Polyclonal
Specificity
Precipitating polyclonal Sheep antiSerum to Guinea Pig IgM, Fc specific.
Form/Format
Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal sheep serum. No foreign proteins added.
Applicable Applications for Sheep anti Guinea Pig IgM (Fc specific) secondary antibody
Immunoelectrophoresis (IEP)
Application Notes
Precipitation assays. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul in a 3 mm diameter center well and 2 ul serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Source
Highly purified normal IgM isolated from pooled guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Cross- reactivity
The antiSerum does not cross-react with any other component of the Guinea Pig Ig system.Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
Reconstitution
Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water.
Preparation and Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation
occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
Related Product Information for Sheep anti Guinea Pig IgM (Fc specific) secondary antibody
The reactivity of the antiserum is restricted to the Fc part of the IgM molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal guinea pig serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No precipitation reaction is obtained with purified IgG, IgA, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in guinea pig serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of guinea pig IgM from serum or plasma.
Product Categories/Family for Sheep anti Guinea Pig IgM (Fc specific) secondary antibody
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Product Notes
The Sheep anti Guinea Pig IgM (Fc specific) (Catalog #AAA571459) is a Secondary Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Sheep anti Guinea Pig IgM (Fc specific) reacts with Guinea Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Sheep anti Guinea Pig IgM (Fc specific) can be used in a range of immunoassay formats including, but not limited to, Immunoelectrophoresis (IEP). Precipitation assays. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul in a 3 mm diameter center well and 2 ul serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Researchers should empirically determine the suitability of the Sheep anti Guinea Pig IgM (Fc specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sheep anti Guinea Pig IgM (Fc specific), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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